| 李廷慧,刘 芳,任志鹏,侯晓彬.肺鳞状细胞癌和腺癌PD-L1蛋白及相关miRNA表达差异的研究[J].,2022,(13):2495-2498 |
| 肺鳞状细胞癌和腺癌PD-L1蛋白及相关miRNA表达差异的研究 |
| Differences of PD-L1 Protein and Related miRNA Expression in Lung Squamous Cell Carcinoma and Adenocarcinoma |
| 投稿时间：2022-01-27 修订日期：2022-02-23 |
| DOI：10.13241/j.cnki.pmb.2022.13.018 |
| 中文关键词: 非小细胞肺癌 鳞癌 腺癌 PD-L1 miRNA |
| 英文关键词: Non-small cell lung cancer Squamous cell carcinomas Adenocarcinoma PD - L1 miRNA |
| 基金项目:全军"十二五"应用基础研究面上项目（CWS11J105） |
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| 中文摘要: |
| 摘要 目的：探讨肺鳞状细胞癌（鳞癌）和腺癌PD-L1蛋白及相关miRNA表达的差异。方法：2019年5月至2020年11月来我院就诊的非小细胞肺癌初治患者纳入本项研究；按照病理类型，将患者分为腺癌组和鳞癌组；H&E染色检测免疫细胞数量；免疫组化检测PD-L1、ki-67、PD-1、CTLA-4和LAG-3的表达；miRNA测序筛选鳞癌和腺癌间差异表达的miRNA。结果：H&E染色结果显示鳞癌组微环境中免疫细胞的数量为86.86±8.96个/高倍视野（HPF），腺癌组的数量为26.29±3.99个/HPF（t=6.173，P＜0.001）；肺鳞癌组微环境免疫细胞PD-1、CTLA-4和LAG-3阳性表达的比例分别为53.71±6.88%、35.29±3.25%和34.43±3.29%，腺癌组阳性表达的比例分别为22.29±3.80%、13.43±2.32%和24.00±1.98%（t=3.997，P=0.002；t=5.476，P＜0.001；t=2.719，P=0.019）；肺鳞癌组患者PD-L1蛋白阳性表达的比例为76.67%，腺癌组的比例为36.67%（P=0.001）；肺鳞癌PD-L1（miR-135、miR-24和miR-30b等）和PD-1（miR-802、miR-155和miR-3127-5p等）相关miRNA的表达均显著高于腺癌。结论：肺鳞癌PD-L1蛋白及相关miRNA的表达、微环境免疫细胞PD-1、CTLA-4和LAG-3阳性比例均显著高于腺癌。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the differences of PD-L1 protein and related miRNA expression between lung squamous cell carcinoma and adenocarcinoma. Methods: From May 2019 to November 2020, newly treated patients with non-small cell lung cancer in our hospital were included in this study. According to the pathological types, the patients were divided into adenocarcinoma group and squamous cell carcinoma group. H&E staining was used to detect the number of immune cells. PD-L1, Ki-67, PD-1, CTLA-4 and LAG-3 expression was detected by immunohistochemistry. The differentially expressed miRNAs between squamous cell carcinoma and adenocarcinoma were screened by miRNA sequencing. Results: H&E staining results showed that the number of microenvironment immune cells in squamous cell carcinoma group was 86.86±8.96/HPF, and that in adenocarcinoma group was 26.29±3.99 /HPF (t=6.173, P<0.001). The positive expression rates of PD-1, CTLA-4 and LAG-3 in microenvironment immune cells of lung squamous cell carcinoma group were 53.71±6.88%, 35.29±3.25% and 34.43±3.29%, respectively, and those of adenocarcinoma group were 22.29±3.80%, 13.43±2.32% and 24.00±1.98% (t=3.997, P=0.002; t=5.476, P<0.001; t=2.719, P=0.019). The positive expression of PD-L1 protein was 76.67% in lung squamous cell carcinoma group and 36.67% in adenocarcinoma group (P=0.001). The expression of miRNAs associated with PD-L1 (miR-135, miR-24 and miR-30b, etc.) and PD-1 (miR-802, miR-155 and miR-3127-5p, etc.) in lung squamous cell carcinoma was significantly higher than that in adenocarcinoma. Conclusion: The expression of PD-L1 protein and related miRNA in lung squamous cell carcinoma, the positive proportion of PD-1, CTLA-4 and LAG-3 in microenvironment immune cells were significantly higher than those in adenocarcinoma. |
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