文章摘要
蔡楚泉,李忆岚,陈 卓,石晓玲,柳鹏程.溃结汤灌肠治疗对湿热夹瘀型溃疡性结肠炎患者肠黏膜屏障功能和外周血单核细胞TLR4/NF-κB信号通路的影响[J].,2024,(3):596-600
溃结汤灌肠治疗对湿热夹瘀型溃疡性结肠炎患者肠黏膜屏障功能和外周血单核细胞TLR4/NF-κB信号通路的影响
Effect of Enema with Kuijie Decoction on Intestinal Mucosal Barrier Function and TLR4/NF-κB Signaling Pathway in Peripheral Blood Mononuclear Cells in Patients with Ulcerative Colitis of Damp Heat with Blood Stasis Type
投稿时间:2023-07-06  修订日期:2023-07-27
DOI:10.13241/j.cnki.pmb.2024.03.038
中文关键词: 溃结汤  溃疡性结肠炎  湿热夹瘀型  肠黏膜屏障功能  TLR4/NF-κB信号通路
英文关键词: Kuijie decoction  Ulcerative colitis  Damp heat with blood stasis type  Intestinal mucosal barrier function  TLR4/NF-κB signaling pathway
基金项目:湖南省卫生健康委科研计划项目(202103031576)
作者单位E-mail
蔡楚泉 长沙市中医医院(长沙市第八医院)消化内科 湖南 长沙 410199 cai19902023@163.com 
李忆岚 长沙市中医医院(长沙市第八医院)消化内科 湖南 长沙 410199  
陈 卓 长沙市中医医院(长沙市第八医院)消化内科 湖南 长沙 410199  
石晓玲 长沙市中医医院(长沙市第八医院)消化内科 湖南 长沙 410199  
柳鹏程 湖南中医药大学研究生院 湖南 长沙 410208  
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中文摘要:
      摘要 目的:观察溃结汤灌肠治疗对湿热夹瘀型溃疡性结肠炎(UC)患者肠黏膜屏障功能和外周血单核细胞Toll样受体4(TLR4)/核因子(NF)-κB信号通路的影响。方法:选择2020年4月~2022年8月期间长沙市中医医院收治的UC活动期湿热夹瘀型患者96例,按照随机数字表法将患者分为实验组(常规西医和溃结汤灌肠治疗,n=48)和对照组(常规西医治疗,n=48)。观察两组患者中医证候积分、改良Baron内镜评分、炎症性肠病问卷(IB-DQ)、血清肠黏膜屏障功能指标[D-乳酸(D-LA)、二胺氧化酶(DAO)、内毒素(LPS)]、TLR4/NF-κB信号通路相关指标。结果:治疗后,两组身热、腹泻粘液脓血便、里急后重、小便短赤、肢体倦怠、胸胁胀痛、腹痛腹胀、肛门灼热、面色晦暗评分均下降,且实验组低于对照组同时间点(P<0.05)。治疗后,两组改良Baron内镜评分下降,且实验组低于对照组同时间点(P<0.05),治疗后,两组IB-DQ评分升高,且实验组高于对照组(P<0.05)。治疗后,两组D-LA、DAO、LPS下降,且实验组低于对照组同时间点(P<0.05)。治疗后,两组TLR4mRNA、NF-κBmRNA下降,且实验组低于对照组同时间点(P<0.05)。结论:溃结汤灌肠治疗对湿热夹瘀型UC患者,可有效改善患者的临床症状,降低中医证候积分,改善肠黏膜屏障功能,调节外周血单核细胞TLR4/NF-κB信号通路。
英文摘要:
      ABSTRACT Objective: To observe the effect of enema with kuijie decoction on intestinal mucosal barrier function and toll-like receptor 4 (TLR4)/nuclear factor (NF) -κB signaling pathway in peripheral blood mononuclear cells of patients with ulcerative colitis (UC) of damp heat with blood stasis type. Methods: 96 patients with active UC of damp heat with blood stasis type who were admitted to Changsha Hospital of Traditional Chinese Medicine from April 2020 to August 2022 were selected, and patients were divided into experimental group (conventional western medicine and enema with kuijie decoction treatment, n=48) and control group (conventional western medicine treatment, n=48) according to the random number table method. The chinese medicine syndrome score, modified Baron endoscopy score, inflammatory bowel disease questionnaire (IB-DQ), serum intestinal mucosal barrier function indexes [D-lactic acid (D-LA), diamine oxidase (DAO), endotoxin (LPS)] and TLR4/NF-κB signaling pathway related indexes were observed in two groups. Results: After treatment, the scores of general fever, diarrhea mucopurulent bloody stool, tenesmus, scanty dark urine, limb burnout, chest and flank distension and pain, abdominal pain and abdominal distension, anal burning and dull complexion in two groups decreased, and those in experimental group were lower than control group at the same time point(P<0.05). After treatment, the modified Baron endoscopy scores in two groups decreased, and experimental group was lower than control group at the same time point(P<0.05), after treatment, the IB-DQ scores in two groups increased, and experimental group was higher than control group(P<0.05). After treatment, D-LA, DAO and LPS in two groups decreased, and those in experimental group were lower than control group at the same time point (P<0.05). After treatment, TLR4 mRNA and NF-κB mRNA in two groups decreased, and those in experimental group were lower than control group at the same time point(P<0.05). Conclusion: Enema with kuijie decoction treatment can effectively improve the clinical symptoms of patients with damp heat with blood stasis type UC, reduce traditional chinese medicine syndrome scores, improve intestinal mucosal barrier function, and regulate TLR4/NF-κB signaling pathway in peripheral blood monocytes.
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