| 刘 格,龙 燕,黄学阳,林鸿国,傅 强.甲状腺乳头状癌组织微小RNA-93-5p、微小RNA-98-5p表达与临床病理特征和增殖、侵袭基因表达的关系研究[J].,2024,(12):2264-2268 |
| 甲状腺乳头状癌组织微小RNA-93-5p、微小RNA-98-5p表达与临床病理特征和增殖、侵袭基因表达的关系研究 |
| Study on the Relationship between the Expression of microRNA-93-5p and microRNA-98-5p in Papillary Thyroid Carcinoma and Clinicopathological Features, Proliferation and Invasion Gene Expression |
| 投稿时间：2024-01-09 修订日期：2024-01-30 |
| DOI：10.13241/j.cnki.pmb.2024.12.011 |
| 中文关键词: 甲状腺乳头状癌 miR-93-5p miR-98-5p 病理特征 增殖基因 侵袭基因 |
| 英文关键词: Papillary thyroid carcinoma miR-93-5p miR-98-5p Clinicopathological features Proliferation gene Invasion gene |
| 基金项目:广东省中医药局科研项目（20191147）；广东省中医药局科研项目（20232033） |
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| 中文摘要: |
| 摘要 目的：研究甲状腺乳头状癌（PTC）组织微小核糖核酸-93-5p（miR-93-5p）、微小RNA-98-5p（miR-98-5p）表达与临床病理特征和增殖、侵袭基因表达的关系。方法：选取2020年10月到2023年10月在广东省中医院行手术切除的PTC患者153例作为研究对象，收集术中切除的癌组织以及癌旁组织。检测并比较癌组织与癌旁组织miR-93-5p、miR-98-5p及增殖基因、侵袭基因mRNA表达水平，分析miR-93-5p及miR-98-5p表达与PTC患者临床病理特征的关系。利用Pearson法分析miR-93-5p、miR-98-5p表达水平与增殖基因、侵袭基因mRNA表达的相关性。结果：癌组织的miR-93-5p表达水平较癌旁组织更高，miR-98-5p表达水平较癌旁组织更低（P<0.05）。miR-93-5p高表达PTC患者TNM分期Ⅲ~Ⅳ期、有淋巴结转移及低分化的比例较miR-93-5p低表达PTC患者更高（P<0.05）。miR-98-5p低表达PTC患者TNM分期Ⅲ~Ⅳ期、有淋巴结转移及低分化的比例较miR-98-5p高表达PTC患者更高（P<0.05）。癌组织的增殖基因程序性细胞死亡因子4（PDCD4）及蛋白磷酸酶4调节亚基 （PP4R1）水平较癌旁组织更低（P<0.05），侵袭基因金属蛋白酶解离素9（ADAM9）及Bcl-6共抑制因子样蛋白（BCORL1）水平较癌旁组织更高（P<0.05）。Pearson法分析结果显示，miR-93-5p表达与增殖基因PDCD4及PP4R1表达水平呈负相关，与侵袭基因ADAM9及BCORL1表达水平呈正相关。miR-98-5p表达水平与增殖基因PDCD4及PP4R1水平呈正相关，与侵袭基因ADAM9及BCORL1表达水平呈负相关。结论：PTC患者癌组织miR-93-5p表达升高，miR-98-5p表达降低，与TNM分期、淋巴结转移及分化程度等临床病理特征有关，还可促进PTC癌细胞增殖、侵袭。 |
| 英文摘要: |
| ABSTRACT Objective: To study the relationship between the expression of micro-93-5p (miR-93-5p) and microRNA-98-5p (miR-98-5p) in papillary thyroid carcinoma (PTC) and clinicopathological features, proliferation and invasion gene expression. Methods: 153 PTC patients who were underwent surgical resection in Guangdong Provincial Hospital of Traditional Chinese Medicine from October 2020 to October 2023 were selected as the research objects, and the intraoperatively resected cancer tissues and adjacent tissues were collected. The expression levels of miR-93-5p, miR-98-5p, proliferation gene and invasion gene mRNA in cancer tissues and adjacent tissues were detected and compared, and the relationship between the expression of miR-93-5p and miR-98-5p and the clinicopathological features of PTC patients were analyzed. The correlation between the expression levels of miR-93-5p and miR-98-5p and the mRNA expression of proliferation genes and invasion genes were analyzed by Pearson method. Results: The expression level of miR-93-5p in cancer tissues was higher than that in adjacent tissues, and the expression level of miR-98-5p was lower than that in adjacent tissues (P<0.05). The proportion of TNM stage III~IV, lymph node metastasis and poor differentiation in PTC patients with high expression of miR-93-5p were higher than those in PTC patients with low expression of miR-93-5p(P<0.05). The proportion of TNM stage III-IV, lymph node metastasis and poor differentiation in PTC patients with low expression of miR-98-5p were higher than those in PTC patients with high expression of miR-98-5p (P<0.05). The levels of proliferation gene programmed cell death factor 4 (PDCD4) and protein phosphatase 4 regulatory subunit (PP4R1) in cancer tissues were lower than those in adjacent tissues (P<0.05), and the levels of invasion gene metalloprotease disintegrin 9(ADAM9) and Bcl-6 co-inhibitor-like protein(BCORL1) were higher than those in adjacent tissues (P<0.05). Pearson analysis showed that, the expression of miR-93-5p was negatively correlated with the expression levels of proliferation genes PDCD4 and PP4R1, and positively correlated with the expression levels of invasion genes ADAM9 and BCORL1. The expression level of miR-98-5p was positively correlated with the levels of proliferation genes PDCD4 and PP4R1, and negatively correlated with the expression levels of invasion genes ADAM9 and BCORL1. Conclusion: The expression of miR-93-5p in cancer tissues of PTC patients is increase, and the expression of miR-98-5p is decrease, which are relate to clinicopathological features such as TNM stage, lymph node metastasis and differentiation degree, and can also promote the proliferation and invasion of PTC cancer cells. |
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