| 侯春霞,卢 强,陈 静,李灵敏,秦 晗,陈 召.NLRP3炎症抑制剂BAY-117082对HSC-2细胞系上皮间质转化过程的影响[J].,2024,(13):2434-2439 |
| NLRP3炎症抑制剂BAY-117082对HSC-2细胞系上皮间质转化过程的影响 |
| Effects of NLRP3 Inflammation Inhibitor BAY-117082 on Epithelial-Mesenchymal Transition in HSC-2 Cell Line |
| 投稿时间：2023-12-21 修订日期：2024-01-24 |
| DOI：10.13241/j.cnki.pmb.2024.13.006 |
| 中文关键词: NLRP3炎症抑制剂 口腔鳞状细胞癌 上皮间质转化 |
| 英文关键词: NLRP3 inflammatory inhibitor Oral squamous cell carcinoma Epithelial-mesenchymal transition |
| 基金项目:陕西省技术创新引导专项（2021QFY01-03） |
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| 中文摘要: |
| 摘要 目的：评估选择性NLRP3 炎症小体抑制剂 BAY-117082对口腔鳞状细胞癌生长及上皮间质转化过程的影响。方法：用HSC-2细胞系在裸鼠舌部构建OSCC模型，将小鼠随机分为A、B、C、D组，其中A组为空白对照组，B、C、D组为实验组，C、D组腹腔注射BAY-117082，剂量分别为2.5 mg/kg和5 mg/kg，将舌部肿瘤、淋巴结和肺转移瘤分别采用免疫组化、PCR、Western-blot实验进行分析。结果：适当剂量的BAY-117082 可以显著抑制口腔鳞状细胞癌HSC-2细胞的生长，且随着BAY-117082浓度的升高，抑制效果更加明显。适当剂量的BAY-117082 可以显著抑制口腔鳞状细胞癌HSC-2细胞中间质细胞标志物N-cadherin的表达，同时促进上皮细胞标志物E-cadherin的表达，且随着BAY-117082浓度的升高，抑制或促进作用越明显。结论：适宜浓度的BAY-117082可以有效抑制HSC-2细胞系的上皮间质转化过程，同时有效抑制原位肿瘤及转移瘤的生长，可以作为OSCC一种有前途的治疗策略。 |
| 英文摘要: |
| ABSTRACT Objective: To evaluate the effect of selective NLRP3 inflammasome inhibitor BAY-117082 on the growth and epithelial mesenchymal transition process of oral squamous cell carcinoma. Methods: An OSCC model was constructed on the tongue of nude mice using HSC-2 cell lines. The mice were randomly divided into group A, B, C, and D. Group A was the blank control group, while groups B, C, and D were the experimental group. Group C and D were intraperitoneally injected with BAY-117082 at doses of 2.5 mg/kg and 5 mg/kg, respectively. The tongue tumors, lymph nodes, and lung metastases were analyzed by immunohistochemistry, PCR, and Western blot. Results: An appropriate dose of BAY-117082 can significantly inhibit the growth of oral squamous cell carcinoma HSC-2 cells and the expression of N-cadherin, an intermediate cell marker, while promoting the expression of E-cadherin, an epithelial cell marker. The effect becomes more pronounced with the increase of BAY-117082 concentration. Conclusion: The appropriate concentration of BAY-117082 can effectively inhibit the epithelial mesenchymal transition process of HSC-2 cell lines and effectively inhibit tumor growth, which can be used as a promising treatment strategy for OSCC. |
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