| 杜鹏云,刘小愉,徐佳宁,王 薇,张成岗.间歇性禁食联合苓桂术甘汤和益生元对非酒精性脂肪肝小鼠的作用研究[J].,2024,(19):3609-3615 |
| 间歇性禁食联合苓桂术甘汤和益生元对非酒精性脂肪肝小鼠的作用研究 |
| Effects of Intermittent Fasting Combined with the Decoction of Ling-gui-zhu-gan and Prebiotics on Mice with Non-alcoholic Fatty Liver Disease |
| 投稿时间：2024-04-27 修订日期：2024-05-24 |
| DOI：10.13241/j.cnki.pmb.2024.19.002 |
| 中文关键词: 非酒精性脂肪肝 肠道菌群 间歇性禁食 苓桂术甘汤 益生元 |
| 英文关键词: Non-alcoholic fatty liver disease Gut microbiota Intermittent fasting Ling-gui-zhu-gan decoction Prebiotics |
| 基金项目:北京中医药大学高层次人才科研启动项目（90011451310015） |
|
| 摘要点击次数: 27 |
| 全文下载次数: 12 |
| 中文摘要: |
| 摘要 目的：探究间歇性禁食联合苓桂术甘汤和益生元对非酒精性脂肪肝病（NAFLD）的治疗效果及内在机制。方法：12周高脂饮食（HFD）喂养建立NAFLD小鼠模型，干预组分别是间歇性禁食组（IF）、禁食联合低浓度苓桂术甘汤组（IFLGL）、禁食联合高浓度苓桂术甘汤组（IFLGH）、禁食联合益生元组（IFpre）、禁食联合益生元和高浓度苓桂术甘汤组（IFLGpre）。检测各组小鼠的体重、血清生化指标、肝脏组织学染色、肠道菌群变化和SREBP-1通路相关基因的表达情况。结果：与模型组相比，5个干预组的小鼠体重，血生化指标丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、胆固醇（CHO）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）均显著降低（P＜0.01）；均缓解了肝脏脂质过量积累，肝组织病理损伤得到改善；与对照组相比，模型组的菌群α多样性指标ACE、Chao1和Simpson指数显著降低（P＜0.05），在IF组、IFLGH组、IFpre组和IFLGpre组干预后显著升高（P＜0.01），且4种干预方式均显著提高了乳酸菌（Ligilactobacillus）和双歧杆菌（Bifidobacteriale）等益生菌的含量；IFLGpre组与其他干预组相比，在体重、肝功能指标ALT和AST，血脂指标TG、CHO和HDL-C，肝脏组织学以及肠道菌群等多项结果中治疗效果更显著，且差异具有统计学意义。5种干预方式均显著下调了肝脏SREBP-1通路中的SREBP-1c和FAS基因的表达（P＜0.01），缓解了肝脏的脂质过量积累。结论：间歇性禁食联合苓桂术甘汤和益生元可以通过调节肠道菌群和SREBP-1通路改善NAFLD，且联合干预的治疗效果更显著。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the therapeutic effects and mechanisms of intermittent fasting combined with Lingguizhugan decoction and prebiotics on non-alcoholic fatty liver disease (NAFLD). Methods: NAFLD mouse models were established using a 12-week high-fat diet (HFD). The intervention groups were: intermittent fasting (IF), IF combined with low-dose Lingguizhugan decoction (IFLGL), IF combined with high-dose Lingguizhugan decoction (IFLGH), IF combined with prebiotics (IFpre), and IF combined with prebiotics and high-dose Lingguizhugan decoction (IFLGpre). Body weight, serum biochemical indices, liver histology, gut microbiota composition, and expression of genes related to the SREBP-1 pathway were measured in each group as parameters. Results: Compared to the model group, all intervention groups showed significant reductions in body weight and serum biochemical indices, including ALT, AST, cholesterol (CHO), triglycerides (TG), HDL-C, and LDL-C (P<0.01). Hepatic lipid accumulation and histopathological damage were notably alleviated in all intervention groups. Compared to the control group, the model group had significantly lower gut microbiota ?琢-diversity indices (ACE, Chao1, Simpson) (P<0.05). However, these indices were increased significantly after intervention in the IF, IFLGH, IFpre, and IFLGpre groups (P<0.01). Additionally, these four interventions significantly enhanced the abundance of beneficial bacteria such as Lactobacillale and Bifidobacteriale. The IFLGpre group demonstrated the most significant improvements in body weight, liver function indices (ALT, AST), lipid profiles (TG, CHO, HDL-C), liver histology, and gut microbiota composition, with statistically significant differences compared to other intervention groups. All interventions significantly downregulated SREBP-1c and FAS gene expression in the liver (P<0.01), reducing hepatic lipid accumulation. Conclusion: Intermittent fasting combined with Lingguizhugan decoction and prebiotics ameliorates NAFLD by modulating gut microbiota and the SREBP-1 pathway. Combined interventions have superior therapeutic effects. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
