| ABSTRACT Objective: To investigate the mechanism of Klotho protein on myocardial ischemia-reperfusion injury in rats. Methods: Sixty male SD rats were selected, and 60 rats were divided into normal control group, ischemia reperfusion injury + saline group, ischemia reperfusion injury + low concentration Klotho protein group, ischemia reperfusion injury + high concentration Klotho protein group, 15 in each group. Treatment was performed separately and the expression of relevant serum and protein was analyzed to contrast apoptosis. Results: The serum IL-6, IL-1β, TNF-α, NF-κB, Iκκβ, caspase9, ROS, MDA, apoptosis: Ischemia-reperfusion injury + normal saline group>ischemia-reperfusion injury + low-concentration Klotho protein group>ischemia-reperfusion injury + high-concentration Klotho protein group>normal control group; Serum IκBα, Klotho protein, Heart tissue Klotho protein, caspase3, Cytochrome C, Bax/Bcl-2 protein, ATP, diphosphate, ATP, SOD: Ischemia-reperfusion injury + normal saline groupConclusion: Klotho protein can maintain the function and structural integrity of myocardial mitochondria, improve myocardial tissue, reduce inflammation and oxidative stress, and reduce cardiomyocyte apoptosis. |
