| 李 霞,陈 浩,杨海阳,王俞卓,王立国.肠源性内毒素血症在大鼠肝肺综合征形成中的作用研究[J].,2025,(18):2897-2902 |
| 肠源性内毒素血症在大鼠肝肺综合征形成中的作用研究 |
| The Role of Intestinal Endotoxemia in the Development of Hepatopulmonary Syndrome in Rats |
| 投稿时间：2025-06-13 |
| DOI：10.13241/j.cnki.pmb.2025.18.003 |
| 中文关键词: 肝肺综合征 肠源性内毒素血症 肿瘤坏死因子α 白细胞介素6 |
| 英文关键词: Hepatopulmonary Syndrome Intestinal endotoxemia TNF-α IL-6 |
| 基金项目:黑龙江省卫生计生委科研课题（2016-127） |
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| 中文摘要: |
| 摘要 目的：探讨肠源性内毒素血症在大鼠肝肺综合征形成中的作用与机制。方法：随机将大鼠分实验组及对照组各10只，通过胆总管结扎法建立大鼠肝肺综合征模型，测定血浆内毒素、TNF-α、IL-6含量；体外分离培养大鼠肺脏微血管内皮细胞，内毒素诱导该细胞活化增殖，检测其TNF-α和IL-6水平。结果：肝肺综合征大鼠血浆内毒素水平明显升高，可诱导细胞因子TNF-α和IL-6产生，引起肺血管扩张及低氧血症形成。结论：肠源性内毒素血症在肝肺综合征形成中作用显著，其可能机制为诱导肺脏微血管内皮细胞产生多种炎症细胞因子，促进肺血管扩张及肝肺综合征的形成，为肝肺综合征的防治策略提供了关键实验依据和潜在靶点。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the role and mechanism of intestinal endotoxemia in the development of hepatopulmonary syndrome in rats. Methods: Rats were randomly divided into Experimental Group (N=10) and Control Group (N=10), and the rat model of Hepatopulmonary Syndrome was established by common bile duct ligation (CBD), and the contents of endotoxin, TNF-α and IL-6 were measured. Rat lung microvascular endothelial cells were isolated and cultured in vitro. Endotoxin induced the activation and proliferation of these cells, and the levels of TNF-β and IL-6 were detected. Results: The level of endotoxin in rats with hepatopulmonary syndrome was significantly elevated, which induced the production of TNF-α and IL-6, causing pulmonary vasodilation and hypoxemia. Conclusion: Intestinal endotoxemia plays an important role in the development of Hepatopulmonary Syndrome, and its possible mechanism is to induce pulmonary microvascular endothelial cells to produce a variety of inflammatory cytokines and to promote the development of pulmonary vasodilation and Hepatopulmonary Syndrome. It provides key experimental evidence and potential targets for the prevention and treatment strategies of hepatopulmonary syndrome. |
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