文章摘要
槲皮素对乳腺癌MCF-7和MDA-MB-435细胞凋亡作用的研究
Study on the effect of quercetin on apoptosis of breast cancer cell MCF-7 and MDA-MB-435
投稿时间:2021-06-01  修订日期:2021-06-01
DOI:
中文关键词: 乳腺癌  槲皮素  细胞增殖  细胞凋亡  Fas  FasL  Bcl-2  Bax  
英文关键词: Breast cancer  Quercetin  Cell proliferation  Apoptosis  Bcl - 2  Bax  Caspase-3
基金项目:2018年重庆市科研机构绩效激励引导专项项目(编号:cstc2018jxj1130063)
作者单位邮编
甘霖 重庆市中医院 400021
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中文摘要:
      目的 探讨槲皮素对乳腺癌MCF-7和MDA-MB-435细胞的凋亡作用,并探讨其作用机制。方法 采用CCK-8法研究槲皮素对乳腺癌MCF-7和MDA-MB-435细胞增殖的作用,分别采用细胞划痕法和Transwell法测定测定对乳腺癌MCF-7和MDA-MB-435细胞迁移和侵袭的影响,采用流式细胞术测定槲皮素对乳腺癌MCF-7和MDA-MB-435细胞凋亡的作用,采用QRT-PCR法和West-blotting测定槲皮素对乳腺癌MCF-7和MDA-MB-435细胞Fas、FasL、Bcl-2和Bax mRNA和蛋白表达的影响。结果 (1)槲皮素(50~200 μmol/L)作用乳腺癌MCF-7和MDA-MB-435细胞 24h、48h和72h均可对其增殖具有显著的抑制作用,并且呈浓度依赖性(P<0.05);(2)细胞划痕实验中槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435细胞划痕宽度较对照组显著增大(P<0.05);(3)Transwell试验中槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435穿膜细胞较对照组显著降低(P<0.05);(4)槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435细胞凋亡率较对照组显著升高(P<0.05);(5)槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435细胞中Bcl-2 mRNA表达较对照组显著降低(P<0.05),Fas、FasL和Bax mRNA较对照组显著升高(P<0.05);(7)槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435细胞中Bcl-2 蛋白较对照组显著降低(P<0.05),Fas、FasL和Bax 蛋白较对照组显著升高(P<0.05)。结论 槲皮素可促进乳腺癌细胞的凋亡,可能与其通过作用Fas/FasL凋亡信号通路而激活外源性凋亡途径以及通过作用Bcl-2凋亡信号通路而激活内源性凋亡途径有关。
英文摘要:
      Objective To study the effects of quercetin on apoptosis of breast cancer cell MCF-7 and MDA-MB-435, and to explore its mechanism. Methods CCK 8 method was used to study the effect of quercetin on proliferation of breast cancer cell MCF - 7 and MDA-MB-435, the scratch method and the Transwell method were respectively used to study the effect of quercetin on migration and invasion of breast cancer cell MCF - 7 and MDA-MB-435. Flow cytometry was used to determine the effect of quercetin on apoptosis in breast cancer cell MCF - 7 and MDA-MB-435. QRT-PCR and West-blotting were used to determine the effect of quercetin on mRNA and protein expression of Fas, FasL, Bcl-2 and Bax in breast cancer cell MCF - 7 and MDA-MB-435. Results (1) Quercetin (5-200 μmol/L) significantly inhibited the proliferation of breast cancer cell MCF-7 and MDA-MB-435 for 24h, 48h and 72h in a concentration dependent manner (P<0.05). (2) Compared with that in the control group, quercetin 50 μmol/L and 100 μmol/L significantly increased cell scratch width (P<0.05). (3) Compared with that in the control group, quercetin 50 μmol/L and 100 μmol/L significantly decreased the number of penetrating cells (P<0.05). (4) Compared with that in the control group, quercetin 50 μmol/L and 100 μmol/L significantly increased the apoptosis rate (P<0.05); (5) Compared with those in the control group, quercetin 50 μmol/L and 100 μmol/L significantly decreased mRNA expression of Bcl-2, while significantly increased mRNA expression of Fas, FasL and Bax (P<0.05). (6) Compared with those in the control group, quercetin 50 μmol/L and 100 μmol/L significantly decreased protein expression of Bcl-2, while significantly increased protein expression of Fas, FasL and Bax (P<0.05). Conclusion Quercetin can promote the apoptosis of the breast cancer cell McF-7 and mda-mb-435, which may be related to the activation of the exogenous apoptosis pathway via Fas/Fasl signal passage and the activation of endogenous apoptosis via Bcl-2 signaling passage.
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