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| 槲皮素对乳腺癌MCF-7和MDA-MB-435细胞凋亡作用的研究 |
| Study on the effect of quercetin on apoptosis of breast cancer cell MCF-7 and MDA-MB-435 |
| 投稿时间：2021-06-01 修订日期：2021-06-01 |
| DOI： |
| 中文关键词: 乳腺癌 槲皮素 细胞增殖 细胞凋亡 Fas FasL Bcl-2 Bax |
| 英文关键词: Breast cancer Quercetin Cell proliferation Apoptosis Bcl - 2 Bax Caspase-3 |
| 基金项目:2018年重庆市科研机构绩效激励引导专项项目（编号：cstc2018jxj1130063） |
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| 中文摘要: |
| 目的 探讨槲皮素对乳腺癌MCF-7和MDA-MB-435细胞的凋亡作用，并探讨其作用机制。方法 采用CCK-8法研究槲皮素对乳腺癌MCF-7和MDA-MB-435细胞增殖的作用，分别采用细胞划痕法和Transwell法测定测定对乳腺癌MCF-7和MDA-MB-435细胞迁移和侵袭的影响，采用流式细胞术测定槲皮素对乳腺癌MCF-7和MDA-MB-435细胞凋亡的作用，采用QRT-PCR法和West-blotting测定槲皮素对乳腺癌MCF-7和MDA-MB-435细胞Fas、FasL、Bcl-2和Bax mRNA和蛋白表达的影响。结果 （1）槲皮素（50~200 μmol/L）作用乳腺癌MCF-7和MDA-MB-435细胞 24h、48h和72h均可对其增殖具有显著的抑制作用，并且呈浓度依赖性（P<0.05）；（2）细胞划痕实验中槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435细胞划痕宽度较对照组显著增大（P<0.05）；（3）Transwell试验中槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435穿膜细胞较对照组显著降低（P<0.05）；（4）槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435细胞凋亡率较对照组显著升高（P<0.05）；（5）槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435细胞中Bcl-2 mRNA表达较对照组显著降低（P<0.05），Fas、FasL和Bax mRNA较对照组显著升高（P<0.05）；（7）槲皮素50 μmol/L和100 μmol/L可使乳腺癌MCF-7和MDA-MB-435细胞中Bcl-2 蛋白较对照组显著降低（P<0.05），Fas、FasL和Bax 蛋白较对照组显著升高（P<0.05）。结论 槲皮素可促进乳腺癌细胞的凋亡，可能与其通过作用Fas/FasL凋亡信号通路而激活外源性凋亡途径以及通过作用Bcl-2凋亡信号通路而激活内源性凋亡途径有关。 |
| 英文摘要: |
| Objective To study the effects of quercetin on apoptosis of breast cancer cell MCF-7 and MDA-MB-435, and to explore its mechanism. Methods CCK 8 method was used to study the effect of quercetin on proliferation of breast cancer cell MCF - 7 and MDA-MB-435, the scratch method and the Transwell method were respectively used to study the effect of quercetin on migration and invasion of breast cancer cell MCF - 7 and MDA-MB-435. Flow cytometry was used to determine the effect of quercetin on apoptosis in breast cancer cell MCF - 7 and MDA-MB-435. QRT-PCR and West-blotting were used to determine the effect of quercetin on mRNA and protein expression of Fas, FasL, Bcl-2 and Bax in breast cancer cell MCF - 7 and MDA-MB-435. Results (1) Quercetin (5-200 μmol/L) significantly inhibited the proliferation of breast cancer cell MCF-7 and MDA-MB-435 for 24h, 48h and 72h in a concentration dependent manner (P<0.05). (2) Compared with that in the control group, quercetin 50 μmol/L and 100 μmol/L significantly increased cell scratch width (P<0.05). (3) Compared with that in the control group, quercetin 50 μmol/L and 100 μmol/L significantly decreased the number of penetrating cells (P<0.05). (4) Compared with that in the control group, quercetin 50 μmol/L and 100 μmol/L significantly increased the apoptosis rate (P<0.05); (5) Compared with those in the control group, quercetin 50 μmol/L and 100 μmol/L significantly decreased mRNA expression of Bcl-2, while significantly increased mRNA expression of Fas, FasL and Bax (P<0.05). (6) Compared with those in the control group, quercetin 50 μmol/L and 100 μmol/L significantly decreased protein expression of Bcl-2, while significantly increased protein expression of Fas, FasL and Bax (P<0.05). Conclusion Quercetin can promote the apoptosis of the breast cancer cell McF-7 and mda-mb-435, which may be related to the activation of the exogenous apoptosis pathway via Fas/Fasl signal passage and the activation of endogenous apoptosis via Bcl-2 signaling passage. |
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