| Objective: The representative modeling methods were evaluated by establishing the sepsis models of Escherichia coli infection type, lipopolysaccharide induced type and host barrier destruction (cecal ligation). Methods: SD rats were gathered and set up as: Escherichia coli group, lipopolysaccharide group, cecal ligation group, control group 1, control group 2, control group 3, with 10 rats in each group. Inflammatory indexes: interleukin-6 (IL-6), procalcitonin (PCT), coagulation function indexes: prothrombin time (PT), activated partial thromboplastin time (APTT), organ dysfunction indexes: creatinine (Cre), alanine aminotransferase (ALT), myocardial calcium t (cTnT) and arterial blood gas analysis indexes: arterial partial pressure of oxygen (PaO2), arterial blood partial pressure of carbon dioxide (PaCO2) level changes were detected within 12h, 24h, 36h, and 48h after modeling. Results: Compared with control group 1, control group 2, and control group 3, within 12h-48h, the IL-6, PCT, PT, APTT, Cre, ALT, cTnT and PaCO2 increased in Escherichia coli group, lipopolysaccharide group and cecal ligation group (P<0.05), the PaO2 level decreased (P<0.05), and the levels of IL-6, PCT, PT, APTT, Cre, ALT, and cTnT in the lipopolysaccharide group and the cecal ligation group were higher than those in the Escherichia coli group (P<0.05), the PaO2 level was lower than that in the Escherichia coli group (P<0.05). Conclusion: All the Escherichia coli modeling, lipopolysaccharide intravenous injection modeling and cecal ligation modeling can replicate the sepsis model, and the severity of the disease in rats established by lipopolysaccharide intravenous injection and cecal ligation is higher than that in rats established by Escherichia coli modeling. |
