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| 妊娠期糖尿病肾功能损害患者血清miR-135b、miR-141表达水平的变化及意义 |
| Changes and significance of serum miR-135b and miR-141 expression levels in patients with renal function impairment in patients with gestational diabetes mellitus |
| 投稿时间：2022-07-22 修订日期：2022-07-22 |
| DOI： |
| 中文关键词: 妊娠期糖尿病 肾功能损害 miR-135b miR-141 孕妇 |
| 英文关键词: Gestational diabetes mellitus Renal function impairment miR-135b miR-141 pregnant woman |
| 基金项目:江苏省卫生与计划生育委员会科研项目（F20170429） |
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| 中文摘要: |
| 目的：探讨血清miR-135b、miR-141在妊娠期糖尿病（GDM）肾功能损害患者中的表达及作用。方法：选取2019年3月至2020年12月在我院就诊的GDM患者200例作为GDM组，根据有无肾功能损伤分为GDM组（n=83）和GDM肾功能损伤组（n=117），再将肾病患者根据尿白蛋白/肌酐比值（UACR）分为轻度65例、中度37例和重度15例。另选择同期在我院产检的正常妊娠孕妇100例为对照组。测定孕妇血清中miR-135b、miR-141水平及肾功能指标，Pearson法分析GDM肾病患者血清miR-135b、miR-141与肾功能指标的相关性。采用非条件Logistic 多元回归分析GDM肾功能损伤的危险因素。结果： GDM肾功能损伤组孕妇血清miR-135b、miR-141表达水平高于GDM组和对照组，GDM组明显高于对照组（P<0.05）。GDM肾功能损伤组肌酐（Scr）、β2-微球蛋白（β2-MG）、UCAR表达水平高于GDM组和对照组，肾小球滤过率（eGFR）低于GDM组和对照组，差异有统计学意义（P<0.05）。血清miR-135b、miR-141表达水平与Scr、β2-MG、UCAR呈正相关（P<0.05），与eGFR呈负相关（P<0.05）。肾功能重度损伤孕妇血清miR-135b、miR-141表达水平明显高于肾功能中度损伤和轻度损伤孕妇，且肾功能中度损伤明显高于轻度损伤（P<0.05）。非条件 Logistic 多元回归分析结果显示，miR-135b、miR-141表达水平是孕妇GDM发生的危险因素（P<0.05）。结论：GDM肾功能损伤患者血清miR-135b、miR-141水平异常升高，且随着肾功能损害的加重其水平逐渐增加，可为GDM肾功能损伤的预测和治疗提供一定参考。 |
| 英文摘要: |
| Objective: To investigate the expression and effect of serum miR-135b and miR-141 in patients with renal function impairment in patients with gestational diabetes mellitus (GDM). Methods: 200 patients with GDM who were treated in our hospital from March 2019 to December 2020 were selected as GDM group. According to whether there was renal function impairment, they were divided into GDM group (n=83) and GDM renal function impairment group (n=117). Then, according to urinary albumin / creatinine ratio (UACR) that patients with nephropathy were divided into mild with 65 cases, moderate with 37 cases and severe with 15 cases. In addition, 100 normal pregnant women who were underwent prenatal examination in our hospital during the same period were selected as the control group. The levels of serum miR-135b, miR-141 and renal function indexes in pregnant women were determined, and the correlation between serum miR-135b, miR-141 and renal function indexes in patients with GDM nephropathy was analyzed by pearson method. Unconditional Logistic multiple regression was used to analyze the risk factors of renal function impairment in GDM. Results: The expression levels of serum miR-135b and miR-141 in pregnant women in the GDM renal function impairment group were higher than those in the GDM group and control group, and GDM group was significantly higher than the control group (P<0.05). The expression levels of creatinine (Scr) and β2-microglobulin (β2-MG), UCAR were higher than those in the GDM group and control group, and the glomerular filtration rate (eGFR) was lower than that in the GDM group and control group, the differences were statistically significant (P<0.05). The expression levels of serum miR-135b, miR-141 were positively correlated with Scr, β2-MG and UCAR (P<0.05), and negatively correlated with eGFR (P<0.05). The expression levels of serum miR-135b and miR-141 in pregnant women with severe renal function impairment were significantly higher than those in pregnant women with moderate and mild renal function impairment, and the moderate renal function impairment was significantly higher than the mild renal function impairment (P<0.05). Unconditional Logistic multiple regression analysis showed that the expression levels of miR-135b and miR-141 were risk factors for the occurrence of GDM in pregnant women (P<0.05). Conclusion: The levels of serum miR-135b and miR-141 in patients with GDM renal function impairment are abnormally elevated, and gradually increase with the aggravation of renal function impairment, and which can providing certain reference for the prediction and treatment of GDM renal function impairment. |
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