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目的：探讨乙型肝炎病毒(HBV)DNA载量与其血清标志物的相关性。方法：运用荧光定量聚合酶链反应(FQ-PCR)、酶联免疫吸附实验(ELISA)分别检测503 例患者HBV-DNA 载量和HBV 血清标志物。根据HBV 血清标志物结果分为大三阳组、小三阳组、少见模式组、抗体阳性及全阴组，比较各组间HBV-DNA的阳性率及定量值。结果：在大三阳组、小三阳组、少见模式组、抗体阳性及全阴组HBV-DNA 的阳性率分别为90%、65.1%、65.2%、2.0%，HBV-DNA 的定量结果(logHBV-DNA) 别为6.32± 1.96、 2.01± 1.68、3.48± 2.52 (抗体阳性及全阴组阳性例数过低，不纳入统计)。大三阳组HBV-DNA的阳性率显著高于小三阳组(P<0. 05)，大三阳组、小三阳组HBV-DNA 的阳性率与少见模式组比较，差异均无统计学意义(P>0.05)，但大三阳组、小三阳组、少见模式组HBV-DNA 的阳性率均显著高于抗体阳性及全阴组(P<0.01)。HBsAg、HBeAg 阳性组HBV-DNA 的阳性率分别显著高于HBsAg 、HBeAg 阴性组(P<0.01)。小三阳组、少见模式组HBV-DNA 载量均显著低于大三阳组(P<0.01)，少见模式组HBV-DNA 载量显著高于小三阳组(P<0.05)。结论：HBV-DNA的阳性率与HBeAg、HBsAg相关；HBV-DNA载量与HBV 血清标志物模式相关。

英文摘要:

Objective:To investigate the correlation of hepatitis B virus (HBV) DNA load with its serum biomarkers.Methods:HBV DNA load and immune markers were detected in 503 case of patients by Fluorescence quantitative polymerase chain reaction (FQ-PCR)and enzyme-linked immunosorbent assay(ELISA). On the basis of the model of hepatitis B virus immune markers, the HBsAg、 HBeAg and Anti-HBc positive group, HBsAg, Anti-HBe and Anti-HBc positive group, infrequent model group, antibody positive or negative group were set up.Results:The positive rate of HBV DNA in HBsAg, HBeAg and Anti-HBc positive group, HBsAg, Anti-HBe and Anti-HBc positive group, infrequent model group, antibody positive or negative group were respectively 90%, 65.1%, 65.2%, 2.0%, and the HBV DNA quantity (logHBV DNA) were 6.32± 1.96, 2.01± 1.68, 3.48± 2.52 (We rejected to analyze the HBV DNA quantity by the course of the positive case in antibody positive or negative group were so deficient.). The positive rate of HBV-DNA in HBsAg, HBeAg, Anti-HBc positive group was higher than that in HBsAg, Anti-HBe, Anti-HBc positive group (P<0.05). The positive rate of HBV-DNA in HBsAg, HBeAg, Anti-HBc positive group and HBsAg,Anti-HBe, Anti-HBc positive group with infrequent model group were compared, no statistical difference was found (P>0.05). But the positive rate of HBV-DNA in HBsAg, HBeAg, Anti-HBc positive group, HBsAg, Anti-HBe, Anti-HBc positive group, and infrequent model group were remarkable higher than those in the antibody positive or negative group (P<0.01). The positive rate of HBV-DNA in HBsAg positive group and HBeAg positive group were remarkable higher than those in the HBsAg negative group and HBeAg negative group (P<0.01). The load of HBV-DNA in HBsAg, Anti-HBe, Anti-HBc positive group and infrequent model group were remarkable lower than that in the HBsAg, HBeAg, Anti-HBc positive group (P<0.01), and the load of HBV-DNA in infrequent model group was remarkable higher than that in the HBsAg, Anti-HBe, Anti-HBc positive group (P<0.05).Conclusion:The positive rate of HBV DNA was related to the HBeAg and HBsAg; theHBV DNA quantity was related to the model of HBV immune markers in serum.

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