| 何倩倩杜子秀△ 何沐臧怡胡振华王菲金拓.新型聚乙烯亚胺衍生物在COS-7 细胞中转染和毒性的研究[J].现代生物医学进展英文版,2011,11(12):2204-2206. |
| 新型聚乙烯亚胺衍生物在COS-7 细胞中转染和毒性的研究 |
| Transfection and Cytotoxicity Studies of Novel PolyethyleneimineDerivative in COS-7 cells |
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| DOI： |
| 中文关键词: PEI 衍生物 COS-7 细胞 转染 细胞毒性 |
| 英文关键词: Polyethyleneimine COS-7 cells Transfection Cytotoxicity |
| 基金项目:国家自然科学基金(200801198) |
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| 中文摘要: |
| 目的：研究以乙二醛为连接剂的聚乙烯亚胺（Polyethyleneimine,PEI）衍生物Polyimine-PEI 对非洲绿猴肾癌细胞COS-7 的转
染活性和细胞毒性的影响。方法：以荧光素酶质粒为报告基因，研究高分子与DNA 的复合物在COS-7 细胞的转染活性，用MTT
方法研究高分子对COS-7 细胞的毒性。结果：COS-7 细胞实验显示，Polyimine-PEI 具有很低细胞毒性，其毒性显著低于
PEI25kDa，同时也具有高效输送质粒的能力。结论：Polyimine-PEI 是一种新型的高效，低毒在基因治疗领域有相当前景的非病毒
载体。 |
| 英文摘要: |
| Objective: To investigate the transfection efficiency and cell cytotoxicity of Polyethyleneimine (PEI) derivative linking
with glyoxal. Methods: Luciferase plasmid was used as the reporter gene to investigate transfection efficiency in COS-7 cells.
Cytotoxicity was detected by MTT method. Results: COS-7 cells transfection and cytotoxicity results suggested that the gene carrier
(Polyimine-PEI) was less cytotoxicity than that of PEI25kDa, and expressed equal transfection efficiency with PEI25kDa and
Lipofectamine 2000. Conclusion: Polyimine-PEI could be a highly efficient and less toxic gene carrier which was promising in gene
therapy. |
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