| 刘薇2 郑强荪1△ 郭万刚1 杨国栋3 卢晓昭3.非诺贝特通过FoxO1 抑制心肌肥大[J].现代生物医学进展英文版,2012,12(8):1449-1451. |
| 非诺贝特通过FoxO1 抑制心肌肥大 |
| Inhibiting Effects of Fenofibrate on Myocardial HypertrophyMediated by FoxO1 |
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| DOI： |
| 中文关键词: 非诺贝特 血管紧张素Ⅱ 心肌肥大 FoxO1 |
| 英文关键词: Fenofibrate Angiotensin II Myocardial hypertrophy FoxO1 |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨非诺贝特(fenofibrate)对血管紧张素Ⅱ(AngⅡ)诱导的肥大心肌细胞的抑制作用及对FoxO1 表达的影响。方法：首
先采用AngⅡ诱导心肌细胞肥大，将细胞分为三组：对照组：未给予任何干预；心肌细胞肥大组：AngⅡ(10-7 mol／L)刺激细胞；治
疗组：先给予fenofibrate (10-5 mol／L)，30min 后AngⅡ(10-7 mol／L)刺激细胞。应用蛋白免疫印迹法(western-blotting) 和实时定量
PCR 法(real time PCR)检测各组细胞中转录因子FoxO1 的蛋白质及mRNA 含量，心肌细胞肥大的判断使用脑钠肽(brain natriuret ic
pepide BNP)。结果：心肌细胞肥大组的FoxO1 表达较对照组明显降低，而治疗组的FoxO1 表达较心肌肥大组明显升高。结论：非
诺贝特可能通过上调FoxO1 表达，从而抑制心肌细胞肥大。 |
| 英文摘要: |
| Objective: To investigate effect of fenofibrateon on myocardial hypertrophy induced by angiotensin II (Ang II) and
transcription factor FoxO1 expression for theoretical bases of preventing and treating myocardial hypertrophy. Methods: H9C2 cells were
divided into 3 groups: nomal control group; hypertrophy group: cells were stimulated by AngⅡ (10-7 mol/L); treatment group：cells were
treated with fenofibrate (10-5 mol/L), 30 min before adding AngⅡ (10-7mol/L). The method of western-blotting and real time PCR were
adopted to detect the FoxO1 expression. Brain natriuretic pepide (BNP) was the symbol of cardiac myocyte hypertrophy. Results: The
mRNA and protein level of FoxO1 decreased significantly in the hypertrophy group compared with that in the normal control and
treatment group. Conclusion: Fenofibrate inhibits cardiac myocyte hypertrophy, upregulates the expression of FoxO1. |
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