| 于烽谢云陈五岭.α- 促黑激素的固相合成研究[J].现代生物医学进展英文版,2012,12(11):2173-2176. |
| α- 促黑激素的固相合成研究 |
| Research on Solid-phase Synthesis of α-MSH |
| |
| DOI： |
| 中文关键词: 固相合成法 α- 促黑激素 多肽 Fmoc 保护策略 |
| 英文关键词: Soild-phase synthesis α-MSH Peptide Fmoc protection strategy |
| 基金项目: |
|
| Hits: 860 |
| Download times: 1983 |
| 中文摘要: |
| 目的：探索α- 促黑激素的合成工艺。方法：采用多肽固相合成法制备α- 促黑激素。以Rink amide-MBHA 树脂为载体、使用
Fmoc 保护策略、TBTU、HOBt、DIEA 为缩合剂体系，最后用TFA、苯甲硫醚、水、苯酚、乙二硫醇混合液将多肽从树脂上切割下来。
结果：合成后的目标多肽产率达64.9%，经过RP-HPLC 纯化纯度可达98%，质谱鉴定显示纯化产物与目标多肽理论相对分子质量
一致。结论：该方法操作方便，反应结果稳定，为固相合成生产α- 促黑激素提供了一种可行的工艺方案。 |
| 英文摘要: |
| Objective: To investigate a way to prepare the peptide via solid-phase synthesis methods. Methods: α-MSH was synthesized
starting from a Rink amide resin with the Fmoc strategy on solid phase. The peptide was coupled in solution of TBTU, Hobt and
DIEA, then cleaved by TFA, benzyl sulfide, water, phenol are ethanedithiol from resin. Result: The yield of synthesized peptide obtained
was 64.9%. The purity of peptide reached 98% after RP-HPLC purification. MS identification proved the peptide prepared was the target
tridecapeptide. Conclusion: This method provides a feasible process of synthesizing α-MSH. |
|
View Full Text
View/Add Comment Download reader |
| Close |
