| 岳芳桦 初明 史萃 肖鹏 冯嘉辉 初正云 陈雪 王若珺 刘晓妍 岑羽捷 谢少华 徐兰 朱蕴兰 裴军 张明波 翟艳君 王月丹.A549肺癌细胞株中癌睾丸抗原的表达及生物学意义研究[J].现代生物医学进展英文版,2014,14(1):1-6. |
| A549肺癌细胞株中癌睾丸抗原的表达及生物学意义研究 |
| Cancer Testis Antigen Expression and Regulation of A549and Biological Meaning |
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| DOI： |
| 中文关键词: 肺癌 癌睾丸抗原 TGF-beta1 表柔比星 细胞凋亡 |
| 英文关键词: Lung cancer Cancer-testis antigen TGF-beta1 Epirubicin Apoptosis |
| 基金项目:国家自然科学基金项目（81172884），国家基础科学人才培养基金（J1030831/ J0108） |
| Author Name | Affiliation | | YUE Fang-hua,CHU Ming,SHI Cui,XIAO Peng, FENG Jia-hui,CHU Zheng-yun,CHEN Xue,WANG Ruo-jun,LIU Xiao-yan,CEN Yu-jie,XIE Shao-hua,XU Lan,ZHU Yun-lan,PEI Jun,ZHANG Ming-bo,ZHAI Yan-jun,WANG Yue-dan | 北京大学医学部基础医学院辽宁中医药大学药学院 |
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| 中文摘要: |
| 目的：癌睾丸抗原（cancer-testis antigen，CTA）是一类主要表达于睾丸组织和癌组织，并具有较强诱导体液和细胞免疫应答
能力的抗原，可以作为抗肿瘤的理想靶分子。本研究探讨了A549 肺癌细胞系中CTA的表达情况及其生物学意义。方法：本研究
中选取了A549 肺癌细胞株，运用RT-PCR的方法检测了其中九种常见CTA 的表达情况，进一步通过AV-PI 双染法观察了表柔
比星，紫杉醇，卡铂，伊立替康，依托泊苷五种化疗药物对A549 细胞的凋亡作用及其调控CTA 表达的情况。另外用转化生长因子
茁1（TGF-茁1）处理A549 细胞，验证了TGF-茁1 促进细胞生长的作用，进一步检测此时CTA 的表达调控。结果：结果显示
MAGEA1，MAGEA3，MAGEC1，HCA661 在A549 细胞系中稳定表达，表柔比星能够强烈诱导细胞发生凋亡，其诱导作用岁浓度
增高而增强。表柔比星下调了MAGEA1，MAGEA3，MAGEC1 的表达，对HCA661 的表达无影响。TGF-beta1 增强了肿瘤细胞增殖
能力，与此同时下调HCA661 的表达，对MAGEA1，MAGEA3，MAGEC1 的表达无影响。结论：MAGEA1，MAGEA3，MAGEC1，
HCA661 在肺癌细胞系A549 中稳定表达。TGF-beta1 作用48 小时，使HCA661 的表达下调，MAGEA1，MAGEA3，MAGEC1 的表达
无变化。表柔比星作用48小时，使MAGEA1，MAGEA3，MAGEC1 的表达下调，HCA661表达无影响，卡铂，紫杉醇，伊立替康，依
托泊苷对CTA 的表达无调控，为进一步明确CTA 的表达调控机制奠定了研究基础，同时为将HCA661 作为临床中诊断与治疗
的新靶点提供了可行性。 |
| 英文摘要: |
| Objective:Cancer-testis antigens（CTA）are a series of antigens which mostly expressed in testis and cancer cells, and
have strong capacity of inducing humoral and cellular immunity. They are promising as vaccine to tumor. In this investigation, we detect
the expression and regulation of CT antigens in A549 cell line. Methods:Nine kinds of CT antigens are detected of their expression in
A549 cell line using the method of RT-PCR. Also the role of TGF-beta1 and five kinds of chemotherapy medicine playing in the regulation
of CT antigens is investigated.Results:The results show that MAGEA1, MAGEA3, MAGEC1, HCA661 express in A549 cell line.
TGF茁1 down-regulates the expression of HCA661, but has no effect on MAGEA1, MAGEA3 and MAGEC1. Epirubicin down-regulates
the expression of MAGEA1, MAGEA3 and MAGEC1, but have no effect on HCA661.Conclusion:MAGEA1, MAGEA3, MAGEC1,
HCA661 stably express in A549 lung cancer cell line. For 48 hours, TGF-茁1 down-regulate the expression of HCA661, MAGEA1,
MAGEA3, MAGEC1, unchanged. Epirubicin down-regulate the expression of MAGEA1, MAGEA3 and MAGEC1, but it does not
affect HCA661. Carboplatin, paclitaxel, irinotecan, etoposide have no CT antigen expression regulation. Our study laid the basis for
further clarification of the mechanism of CTA expression regulation and provided feasibility of HCA661 as a new target for clinical
diagnosis and treatment. |
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