汪东剑 张晓云 李志晋 江丁 韩香妮.HBV-DNA 与乙型肝炎血清标志物的相关性分析[J].现代生物医学进展英文版,2014,14(3):444-447. |
HBV-DNA 与乙型肝炎血清标志物的相关性分析 |
Analysis of the Correlation between SerumImmune Markers of Hepatitis BVirus and HBV-DNA |
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DOI: |
中文关键词: HBV-DNA 乙型肝炎 乙型肝炎表面抗原 乙型肝炎e 抗原 |
英文关键词: HBV-DNA Hepatitis B Hepatitis B surface antigens Hepatitis B e antigens |
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中文摘要: |
目的:探讨乙型肝炎病毒(HBV)DNA载量与其血清标志物的相关性。方法:运用荧光定量聚合酶链反应(FQ-PCR)、酶联免疫
吸附实验(ELISA)分别检测503 例患者HBV-DNA 载量和HBV 血清标志物。根据HBV 血清标志物结果分为大三阳组、小三阳
组、少见模式组、抗体阳性及全阴组,比较各组间HBV-DNA的阳性率及定量值。结果:在大三阳组、小三阳组、少见模式组、抗体
阳性及全阴组HBV-DNA 的阳性率分别为90%、65.1%、65.2%、2.0%,HBV-DNA 的定量结果(logHBV-DNA) 别为6.32± 1.96、
2.01± 1.68、3.48± 2.52 (抗体阳性及全阴组阳性例数过低,不纳入统计)。大三阳组HBV-DNA的阳性率显著高于小三阳组(P<0.
05),大三阳组、小三阳组HBV-DNA 的阳性率与少见模式组比较,差异均无统计学意义(P>0.05),但大三阳组、小三阳组、少见模式
组HBV-DNA 的阳性率均显著高于抗体阳性及全阴组(P<0.01)。HBsAg、HBeAg 阳性组HBV-DNA 的阳性率分别显著高于HBsAg
、HBeAg 阴性组(P<0.01)。小三阳组、少见模式组HBV-DNA 载量均显著低于大三阳组(P<0.01),少见模式组HBV-DNA 载量
显著高于小三阳组(P<0.05)。结论:HBV-DNA的阳性率与HBeAg、HBsAg相关;HBV-DNA载量与HBV 血清标志物模式相关。 |
英文摘要: |
Objective:To investigate the correlation of hepatitis B virus (HBV) DNA load with its serum biomarkers.Methods:HBV DNA load and immune markers were detected in 503 case of patients by Fluorescence quantitative polymerase chain reaction
(FQ-PCR)and enzyme-linked immunosorbent assay(ELISA). On the basis of the model of hepatitis B virus immune markers, the HBsAg、
HBeAg and Anti-HBc positive group, HBsAg, Anti-HBe and Anti-HBc positive group, infrequent model group, antibody positive or negative
group were set up.Results:The positive rate of HBV DNA in HBsAg, HBeAg and Anti-HBc positive group, HBsAg, Anti-HBe and
Anti-HBc positive group, infrequent model group, antibody positive or negative group were respectively 90%, 65.1%, 65.2%, 2.0%, and
the HBV DNA quantity (logHBV DNA) were 6.32± 1.96, 2.01± 1.68, 3.48± 2.52 (We rejected to analyze the HBV DNA quantity by
the course of the positive case in antibody positive or negative group were so deficient.). The positive rate of HBV-DNA in HBsAg,
HBeAg, Anti-HBc positive group was higher than that in HBsAg, Anti-HBe, Anti-HBc positive group (P<0.05). The positive rate of
HBV-DNA in HBsAg, HBeAg, Anti-HBc positive group and HBsAg,Anti-HBe, Anti-HBc positive group with infrequent model group
were compared, no statistical difference was found (P>0.05). But the positive rate of HBV-DNA in HBsAg, HBeAg, Anti-HBc positive
group, HBsAg, Anti-HBe, Anti-HBc positive group, and infrequent model group were remarkable higher than those in the antibody positive
or negative group (P<0.01). The positive rate of HBV-DNA in HBsAg positive group and HBeAg positive group were remarkable
higher than those in the HBsAg negative group and HBeAg negative group (P<0.01). The load of HBV-DNA in HBsAg, Anti-HBe,
Anti-HBc positive group and infrequent model group were remarkable lower than that in the HBsAg, HBeAg, Anti-HBc positive group
(P<0.01), and the load of HBV-DNA in infrequent model group was remarkable higher than that in the HBsAg, Anti-HBe, Anti-HBc
positive group (P<0.05).Conclusion:The positive rate of HBV DNA was related to the HBeAg and HBsAg; theHBV DNA quantity was
related to the model of HBV immune markers in serum. |
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