| 张彦丽,代亚丽,陈亚兰,靳 娇,潘 勋,王文娟.利用转盘共聚焦显微镜进行快速实验的新方法[J].现代生物医学进展英文版,2019,19(19):3784-3788. |
| 利用转盘共聚焦显微镜进行快速实验的新方法 |
| New Methods for Rapid Experiment using Spinning Disk Confocal Microscope |
| Received:February 28, 2019 Revised:March 23, 2019 |
| DOI：10.13241/j.cnki.pmb.2019.19.043 |
| 中文关键词: 激光共聚焦显微镜 转盘共聚焦显微镜 快速成像 |
| 英文关键词: Laser scanning confocal microscopy Spinning disk confocal microscopy Fast images |
| 基金项目: |
| Author Name | Affiliation | E-mail | | ZHANG Yan-li | 1 School of Life Sciences, Tsinghua University, Beijing, 100084, China
2 Technology Center For Protein Sciences, Tsinghua University, Beijing, 100084, China | huihui78600@mail.tsinghua.edu.cn | | DAI Ya-li | 1 School of Life Sciences, Tsinghua University, Beijing, 100084, China
2 Technology Center For Protein Sciences, Tsinghua University, Beijing, 100084, China | | | CHEN Ya-lan | 1 School of Life Sciences, Tsinghua University, Beijing, 100084, China
2 Technology Center For Protein Sciences, Tsinghua University, Beijing, 100084, China | | | JIN Jiao | Center of Beijing Biomedical Analysis,Tsinghua University, Beijing, 100084, China | | | PAN Xun | Center of Beijing Biomedical Analysis,Tsinghua University, Beijing, 100084, China | | | WANG Wen-juan | 1 School of Life Sciences, Tsinghua University, Beijing, 100084, China
2 Technology Center For Protein Sciences, Tsinghua University, Beijing, 100084, China | |
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| 中文摘要: |
| 摘要：转盘共聚焦显微镜是快速激光共聚焦显微镜的一种，与传统的激光共聚焦显微镜相比具有一些相同点，也有其特有的优势。本文主要介绍转盘共聚焦显微镜的基本原理及如何利用转盘共聚焦显微镜进行快速实验及应用实例，并与传统激光共聚焦显微镜进行比较。转盘共聚焦显微镜具有速度快、灵敏度高、对样品光损伤和光淬灭程度低、操作灵活简单，是随着实验技术发展使用越来越广泛的实验仪器。 |
| 英文摘要: |
| ABSTRACT: Spinning disk confocal microscope is a kind of fast laser confocal microscope. Compared with traditional point Laser scanning confocal microscope, spinning disk confocal microscope has some common points and unique advantages. This paper mainly introduces the basic principle of spinning disk confocal microscope and how to use spinning disk confocal microscope to take out rapid experiments and application examples, and compares it with common point scanning confocal microscope. It is concluded that the spinning disk confocal microscope has the advantages of fast speed, high sensitivity, low degree of light damage and low bleaching to the sample, flexible and simple operation, and is an experimental instrument that is more and more widely used with the development of experimental technology. |
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