| 周翠红,薛姗姗,刘江正,王化宁,顾婷婷,彭正午.天麻素对镉诱导的星形胶质细胞损伤的保护效应[J].现代生物医学进展英文版,2020,(6):1015-1021. |
| 天麻素对镉诱导的星形胶质细胞损伤的保护效应 |
| Protective Effect of Gastrodin on Cadmium-induced Injury of Astrocytes of Mice |
| Received:August 23, 2019 Revised:September 18, 2019 |
| DOI：10.13241/j.cnki.pmb.2020.06.004 |
| 中文关键词: 镉 星形胶质细胞 GDNF Nrf2 HO-1 SOD-1 |
| 英文关键词: Cadmium Gastrodin GDNF Nrf2 HO-1 SOD-1 |
| 基金项目:国家自然科学基金项目（81630032；81401109）；国家科技支撑计划项目（2016YFC1307100） |
| Author Name | Affiliation | E-mail | | ZHOU Cui-hong | Department of Psychosomatic, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | zch4610@126.com | | XUE Shan-shan | Department of Psychosomatic, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | LIU Jiang-zheng | Department of toxicology, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | WANG Hua-ning | Department of Psychosomatic, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | GU Ting-ting | Department of Anesthesia, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | PENG Zheng-wu | Department of Psychosomatic, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | |
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| 中文摘要: |
| 摘要 目的：探讨氯化镉(CdCl2)和天麻素(GAS)对小鼠星形胶质细胞活力及神经营养因子GDNF和抗氧化基因Nrf2，HO-1，SOD-1表达的影响。方法：首先，给予体外培养的小鼠星形胶质细胞不同浓度的CdCl2 (Con，2.5 μM，5 μM，10 μM，20 μM）处理24 h或48 h，随后检测细胞活力筛选出造成星形胶质细胞损伤的CdCl2浓度和时间。然后使用上述筛选的CdCl2作用浓度(5 μM)构建星形胶质细胞损伤的同时再给予不同浓度的天麻素(0，20 μg/mL，30 μg/mL，40 μg/mL, 50 μg/mL)处理24 h或48 h, 随后检测细胞活力并提取细胞RNA检测其caspase3，GDNF(胶质源性神经营养因子Glial cell-derived neurotrophic factor，GDNF)和Nrf2(Nuclear factor erythroid2-related factor2)，HO-1(Heme oxygenase 1)，SOD-1(superoxide dismutase 1)等抗氧化基因的mRNA表达的变化。结果：(1) 2.5 μM CdCl2处理24 h后星形胶质细胞活力已经有明显下降(P<0.05)，5 μM CdCl2处理24 h后，星形胶质细胞活力显著下降(P<0.01)；(2) CdCl2浓度越大，细胞损伤严重；(3) 一定浓度的天麻素处理可以缓解CdCl2造成的星形胶质损伤，恢复其细胞活力，下调caspase3 mRNA水平；(4) CdCl2下调了星形胶质细胞的GDNF, Nrf2, HO-1和SOD-1的mRNA水平，天麻素可以抑制CdCl2对上述基因的mRNA水平的调节作用，且浓度越高调节作用越强。结论：天麻素可能通过调节小鼠星形胶质细胞的GDNF, Nrf2, HO-1和SOD-1基因表达缓解CdCl2导致的细胞损伤。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of cadmium and gastrodin on cell viability and the effect on the expression level of GDNF, Nrf2, HO-1 and SOD-1 of astrocytes from the hippocampus of mice. Methods: First, cultured hippocampal astrocytes from new-born (one day old) mice were divided into control and cadmium groups: control group was treated with medium(5%FBS+DMEM) and cadmium treatment groups were treated with 2.5, 5, 10 or 20 μM CdCl2 for 24 or 48 hours respectively. Then cell viability was measured by CCK-8 and the appropriate concentration and time point (5 μM, 24 h) of CdCl2 to induce cell injury were determined. After that, cultured astrocytes were divided into control, sham and GAS groups: control group was treated with medium(5%FBS+DMEM), sham group was treated with 5 μM CdCl22 for 24 h, GAS groups were treated with 5 μM CdCl2 and 20 μg/mL, 30 μg/mL, 40 μg/mL, 50 μg/mL gastrodin for 24 h respectively at the same time. Then cell viability was measured by CCK-8 and total RNA was extracted from cells and the mRNA levels of GDNF, Nrf2, HO-1 and SOD-1 were measured by Real-time PCR. Results: (1) Compared with the control, CdCl2 significantly decreased the cell viability of astrocytes; (2) The effect of isoflurane revealed a dose effect---compared with 2.5 μM group, cells treated with 5.0-20 μM of CdCl2 suffered more influence;(3) 5μM CdCl2 treated 24 h significantly down-regulated the mRNA levels of GDNF, Nrf2, HO-1 and SOD-1 mRNA level in the astrocytes; (4) Gastrodin administration restored the cell viability and expression of caspase3, GDNF, Nrf2, HO-1 and SOD-1 in the astrocytes post-CdCl2 exposures. Conclusion: Gastrodin administration restored the decreased cell viability and reversed the disturbed expression of GDNF, Nrf2, HO-1 and SOD-1 in astrocytes exposed to CdCl2. |
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