| 陈建欢,王 蕾,马 蓉,陈复刚,马彩玲.DDX1基因下调对宫颈鳞癌SiHa细胞增殖、凋亡、侵袭和迁移能力的影响[J].现代生物医学进展英文版,2024,(19):3628-3632. |
| DDX1基因下调对宫颈鳞癌SiHa细胞增殖、凋亡、侵袭和迁移能力的影响 |
| Effects of Dead-box 1 Gene Down-regulation on the Proliferation, Apoptosis, Invasion and Migration of Cervical Squamous Cell Carcinoma SiHa Cells |
| Received:February 08, 2024 Revised:February 28, 2024 |
| DOI：10.13241/j.cnki.pmb.2024.19.005 |
| 中文关键词: DDX1基因 宫颈鳞癌 增殖 凋亡 侵袭 转移 |
| 英文关键词: DDX1 Cervical squamous cell carcinoma Proliferation Apoptosis Migration Invasion |
| 基金项目:国家自然科学基金项目（82260613）；省部共建中亚高发病成因与防治国家重点实验室开放课题（SKL-HIDCA-2019-32；SKL-HIDCA-2022-2） |
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| 中文摘要: |
| 摘要 目的：探讨下调DEAD-box解旋酶1（DDX1）基因对宫颈鳞癌SiHa细胞增殖、凋亡、转移及侵袭能力的影响。方法：siRNA转染构建DDX1低表达SiHa细胞系、和阴性对照组，用RT-qPCR和Westem Blot检测DDX1的mRNA 和蛋白表达水平。应用CCK8法、流式细胞技术、Transwell实验分别检测DDX1低表达对 SiHa 细胞增殖、凋亡、转移和侵袭能力的影响。结果：（1）mRNA水平检测显示：下调DDX1，Si-DDX1组中DDX1表达均低于Si-NC组，差异有统计学意义（P<0.05）。蛋白水平检测显示，Si-DDX1组中DDX1表达低于Si-NC组，差异有统计学意义（P<0.05）。（2）下调DDX1后，Si-DDX1组细胞的增殖活性较 Si-NC 组升高，Si-DDX1组的凋亡率较 Si-NC 组降低，差异有统计学意义（P<0.05）。（3）下调DDX1后，Si-DDX1组细胞转移率和侵袭率均较 Si-NC组增高，差异有统计学意义（P<0.05）。结论：DDX1的表达受到抑制后，宫颈鳞癌SiHa细胞的增殖能力增强、凋亡能力减弱，转移和侵袭能力增强。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the impact of downregulating DDX1 gene on growth, apoptosis, metastasis, and invasion of SiHa cells in cervical cancer. Methods: A DDX1-low-expression SiHa cell line was established via siRNA transfection, alongside a negative control group. The mRNA and protein expression levels of DDX1 were assessed using RT-qPCR and Western Blot analysis, respectively. Additionally, CCK8 assay, flow cytometry analysis, and Transwell assay were conducted to evaluate the effect of decreased DDX1 expression on proliferation, apoptosis, metastasis, and invasion of SiHa cells. Results: (1) Analysis of mRNA levels revealed significantly lower expression of DDX1 in the Si-DDX1 group compared to the Si-NC group(P<0.05). Similarly, analysis at the protein level demonstrated a statistically significant decrease in DDX1 expression in the Si-DDX1 group compared to the Si-NC group(P<0.05). (2) Down-regulation of DDX1 resulted in increased cellular proliferation activity in the Si-DDX1 group compared to the Si-NC group (P<0.05), while exhibiting a lower rate of apoptosis (P<0.05). (3) Decreased expression of DDX1 led to higher rates of metastasis and invasion in the Si-DDX1 group compared to the Si-NC group with statistical significance (P<0.05). Conclusion: Inhibition or reduction of DDX1 expression enhances cellular proliferation ability while weakening apoptotic capability; additionally promoting metastatic potential and invasive abilities within SiHa cells. |
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