| 高龙飞,张景丽,吴晓杰,吴会芳,段晨初,康军聪,张英谦.血清miR-21、S1P与难治性肺炎支原体肺炎患儿并发肝损伤的关系研究[J].现代生物医学进展英文版,2024,(19):3651-3653. |
| 血清miR-21、S1P与难治性肺炎支原体肺炎患儿并发肝损伤的关系研究 |
| Study on the Relationship between Serum miR-21, S1P and Liver Injury in Children with Refractory Mycoplasma Pneumoniae Pneumonia |
| Received:March 21, 2024 Revised:April 17, 2024 |
| DOI：10.13241/j.cnki.pmb.2024.19.011 |
| 中文关键词: 肺炎支原体肺炎 微小核糖核酸-21 1-磷酸鞘氨醇 肝损伤 |
| 英文关键词: Mycoplasma pneumoniae pneumonia MicroRNA-21 Sphingosine-1-phosphate Liver injury |
| 基金项目:河北省卫生健康委科研基金项目（20211381） |
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| 中文摘要: |
| 摘要 目的：探讨血清微小核糖核酸-21（miR-21）、1-磷酸鞘氨醇（S1P）与难治性肺炎支原体肺炎（RMPP）患儿并发肝损伤的关系。方法：根据丙氨酸氨基转移酶（ALT）水平将270例RMPP患儿分为肝损伤组（≥40 U/L，39例），无肝损伤组（＜40 U/L，231例），并将肝损伤患儿分为轻度肝损伤组（40 U/L～＜200 U/L，20例）、中度肝损伤组（200 U/L～＜400 U/L，13例）和重度肝损伤组（≥400 U/L，6例）。采用实时荧光定量聚合酶链式反应（RT-PCR）检测miR-21水平，酶联免疫吸附法检测S1P水平。通过单因素及多因素Logistic回归分析RMPP患儿并发肝损伤的影响因素。结果：与对照组比较，RMPP组血清miR-21水平升高，S1P水平降低（P＜0.05）。无肝损伤组、轻度肝损伤组、中度肝损伤组、重度肝损伤组血清miR-21水平依次升高，S1P水平依次降低（P＜0.05）。经单因素及多因素Logistic回归分析显示热程延长、血清CRP水平升高、血清miR-21水平升高为RMPP患儿并发肝损伤的危险因素，血清S1P水平升高为保护因素（P＜0.05）。结论：血清miR-21水平升高、S1P水平降低与RMPP患儿并发肝损伤及其程度有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between serum microRNA-21 (miR-21), sphingosine-1-phosphate (S1P) and liver injury in children with refractory mycoplasma pneumoniae pneumonia (RMPP). Methods: 270 children with RMPP were divided into liver injury group (≥40 U/L, 39 cases) and non liver injury group (<40 U/L, 231 cases) according to the level of alanine aminotransferase (ALT), and children with liver injury were divided into mild liver injury group (40 U/L~< 200 U/L, 20 cases ), moderate liver injury group (200 U/L~< 400 U/L, 13 cases) and severe liver injury group (≥400 U/L, 6 cases). The level of miR-21 was detected by real-time fluorescence quantitative polymerase chain reaction (RT-PCR), and the level of S1P was detected by enzyme-linked immunosorbent assay. The influencing factors of liver injury in children with RMPP were analyzed by univariate and multivariate logistic regression. Results: Compared with control group, the serum miR-21 level in RMPP group was increased, and the S1P level was decreased (P<0.05). The levels of serum miR-21 in non liver injury group, mild liver injury group, moderate liver injury group and severe liver injury group increased in turn, and the levels of S1P decreased in turn(P<0.05). Univariate and multivariate logistic regression analysis showed that prolonged fever, elevated serum CRP level and elevated serum miR-21 level were risk factors for liver injury in children with RMPP, and elevated serum S1P level was a protective factor (P<0.05). Conclusion: The increase of serum miR-21 level and the decrease of S1P level are related to the degree of liver injury in children with RMPP, and serum miR-21 combined with S1P level has a high predictive value for liver injury in children with RMPP. |
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