| 刘春霞,马小龙,魏云莉,尹 婷,马金海.百日咳患儿血清PT-IgG、IL-17、IFN-γ水平及与肠黏膜屏障功能和体液免疫的关系[J].现代生物医学进展英文版,2024,(19):3719-3722. |
| 百日咳患儿血清PT-IgG、IL-17、IFN-γ水平及与肠黏膜屏障功能和体液免疫的关系 |
| Serum PT-IgG, IL-17 and IFN-γ Levels in Children with Pertussis and Their Relationship with Intestinal Mucosal Barrier Function and Humoral Immunity |
| Received:May 05, 2024 Revised:May 30, 2024 |
| DOI：10.13241/j.cnki.pmb.2024.19.031 |
| 中文关键词: 百日咳 PT-IgG IL-17 IFN-γ 肠黏膜屏障功能 体液免疫 |
| 英文关键词: Pertussis PT-IgG IL-17 IFN-γ Intestinal mucosal barrier function Humoral immunity |
| 基金项目:宁夏回族自治区自然科学基金项目（2023AAC03550） |
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| 中文摘要: |
| 摘要 目的：分析百日咳患儿血清百日咳毒素免疫球蛋白G（PT-IgG）、白介素-17（IL-17）、干扰素γ（IFN-γ）水平及与肠黏膜屏障功能和体液免疫的关系。方法：选取2020年2月至2023年9月宁夏医科大学总医院儿科收治的140例百日咳患儿（百日咳组）及同期持续性咳嗽≥2周但病原学未检出百日咳鲍特菌的80例儿童（对照组）为研究对象，采用酶联免疫吸附试验测定所有患儿血清PT-IgG、白介素-17（IL-17）、干扰素γ（IFN-γ），同时评估其肠黏膜屏障功能[晚期糖基化终末产物（AGEs）、二胺氧化酶（DAO）、内毒素（ETX）]及体液免疫[免疫球蛋白A（IgA）、免疫球蛋白G（IgG）、免疫球蛋白M（IgM）、补体C3、补体C4]水平，分析百日咳患儿PT-IgG、IL-17、IFN-γ水平与肠黏膜屏障功能、体液免疫指标的相关性。结果：百日咳组患儿血清PT-IgG阳性率为35.00%（49/140）；百日咳组血清IL-17、IFN-γ、AGEs、DAO、ETX水平高于对照组，百日咳组血清IgA、IgG、IgM、补体C3、补体C4水平低于对照组（P＜0.05）；百日咳患儿PT-IgG阳性率与IL-17、IFN-γ、AGEs、ETX水平呈负相关，PT-IgG阳性率与IgA、IgM、补体C3水平呈正相关，IL-17、IFN-γ与AGEs、ETX呈正相关，IL-17、IFN-γ与IgA、补体C3水平呈负相关，IL-17与IFN-γ水平呈正相关（P＜0.05）。结论：百日咳患儿血清PT-IgG阳性率低，而IL-17、IFN-γ水平较高，且三者均与肠黏膜屏障功能、体液免疫有一定关系，对其进行检测可为临床决策提供依据。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the serum pertussis toxin immunoglobulin G (PT-IgG), IL-17 and IFN-γ levels in children with pertussis, and their relationship with intestinal mucosal barrier function and humoral immunity. Methods: A total of 140 children with pertussis (pertussis group) who were admitted to the Department of Pediatrics at General Hospital of Ningxia Medical University from February 2020 to September 2023, and 80 children (control group) with 2 weeks or longer duration of persistent cough but without Bordetella pertussis were selected as the study subjects at the same time. Serum PT-IgG, interleukin-17 (IL-17) and interferon-gamma (IFN-γ) levels in all subjects were measured by enzyme-linked immunosorbent assay. Intestinal mucosal barrier function [advanced glycation end products (AGEs), diamine oxidase (DAO) and endotoxin (ETX)] and humoral immunity [immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), complement C3 and complement C4] were evaluated. The correlation between the levels of PT-IgG, IL-17 and IFN-γ and intestinal mucosal barrier function, humoral immunity in children with pertussis was analyzed. Results: Serum PT-IgG positive rate in the pertussis group was 35.00% (49/140). Serum IL-17, IFN-γ, AGEs, DAO, and ETX levels in the pertussis group were higher than those in the control group. Serum IgA, IgG, IgM, complement C3, and complement C4 levels in the pertussis group were lower than those in the control group (P<0.05). PT-IgG positive rate was negatively correlated with the levels of IL-17, IFN-γ, AGEs and ETX in children with pertussis. PT-IgG positivity rate was positively correlated with the levels of IgA, IgM and complement C3. IL-17 and IFN-γ were positively correlated with AGEs and ETX. IL-17 and IFN-γ were negatively correlated with IgA and complement C3. IL-17 was positively correlated with IFN-γ (P<0.05). Conclusion: Serum PT-IgG positive rate is low, while the levels of IL-17 and IFN-γ are relatively high in children with pertussis. The three are related to intestinal mucosal barrier function and humoral immunity. Detecting these indicators can provide a basis for clinical decision-making. |
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