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| PHB2在糖尿病肾病中的表达及临床意义 |
| The expression and clinical significance of PHB2 in diabetic kidney disease* |
| 投稿时间：2025-04-18 修订日期：2025-04-24 |
| DOI： |
| 中文关键词: 糖尿病肾病 PHB2 肾组织活检 免疫组织化学染色 |
| 英文关键词: Diabetic kidney disease Prohibitin 2 Renal biopsy Immunohistochemistry |
| 基金项目:PHB2在高原环境低氧性肺动脉高压肺血管重塑中的作用及机制研究。 |
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| 中文摘要: |
| 目的：探讨抗增殖蛋白2(Prohibitin 2,PHB2)在糖尿病肾病(Diabetic Kidney Disease,DKD)患者肾组织中的表达及临床价值。方法：收集2015年3月至2024年5月期间经肾穿刺活检确诊为糖尿病肾病且符合纳入标准的16例患者(DKD组),同时选取行肾切除术的肾肿瘤患者部分正常肾组织20例为对照组(NC组)。采用多重染色技术评估两组样本：DKD组进行HE染色、PAS染色、Masson染色、PASM染色,NC组进行HE染色和PAS染色。采用免疫组织化学技术半定量分析两组样本中PHB2蛋白的表达差异,并采用Pearson或Spearman相关分析评估其与临床指标(糖化血红蛋白、血肌酐、胱抑素C、血尿素氮、尿微量白蛋白、24小时尿蛋白定量及估算肾小球滤过率)的关系。对显著相关指标进一步进行一元线性回归分析。结果: 1.DKD组肾组织呈现典型的糖尿病肾病病理改变,包括肾小球肥大、系膜基质增生、基底膜增厚及间质炎症细胞浸润等。2.PHB2在肾小管中表达,且DKD组PHB2表达显著低于NC组(P<0.05)。3.相关性分析显示,糖尿病肾病中PHB2的表达水平与糖化血红蛋白、血肌酐、胱抑素C和血尿素氮呈负相关(P<0.05),而与估算肾小球滤过率呈正相关 ( P<0.05)。4.逻辑回归分析显示：PHB2表达是血肌酐、胱抑素C及血尿素氮的负相关因素,同时也是eGFR的正相关因素。结论：PHB2在糖尿病肾病患者肾组织中表达显著降低。PHB2低表达可能加重糖代谢紊乱、肾功能损伤及蛋白尿。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression and clinical significance of Prohibitin 2 (PHB2) in the kidney tissue of patients with Diabetic Kidney Disease (DKD). Method(s): From March 2015 to May 2024, samples were collected from 16 patients diagnosed with diabetic nephropathy through renal biopsy, who met the inclusion criteria (referred to as the DKD group). Additionally, 20 patients with renal tumors undergoing nephrectomy, who had partially normal kidney tissue, were selected to serve as the control group (NC group).Multiple staining techniques were employed to evaluate the tissue samples from both groups. The DKD group samples were stained with hematoxylin and eosin (HE), periodic acid-Schiff (PAS), Masson's trichrome, and PASM, while the NC group samples were stained with HE and PAS.Immunohistochemistry was utilized to analyze the differences in PHB2 protein expression between the two groups. Pearson or Spearman correlation methods were applied for statistical analysis.. Result(s): 1. In the DKD group, typical pathological changes associated with diabetic nephropathy, such as glomerular hypertrophy, mesangial matrix hyperplasia, thickening of the basement membrane, and infiltration of interstitial inflammatory cells, were observed in renal tissue. 2. PHB2 expression was detected in renal tubules, and its levels in the DKD group were found to be significantly lower than those in the NC group (P < 0.05). 3. Correlation analysis showed that the expression level of PHB2 in diabetic nephropathy was negatively correlated with HbA1c, serum creatinine, cystatin C, and blood urea nitrogen (P < 0.05). Conversely, it was positively correlated with the estimated glomerular filtration rate (eGFR) (P < 0.05). 4. Logistic regression analysis revealed that PHB2 expression was identified as a negative correlation factor for serum creatinine, cystatin C, and blood urea nitrogen, while it was established as a positive correlation factor for eGFR. eGFR. Conclusion(s): In patients with diabetic nephropathy, the expression of PHB2 in renal tissue significantly decreases. This reduction in PHB2 levels closely correlates with glucose metabolism and renal function. Low levels of PHB2 may worsen glucose metabolism disorders, renal function damage, and proteinuria. Therefore, PHB2 serves as a potential biomarker for assessing prognosis and offers new insights into the treatment of diabetic kidney disease. |
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