| 毛天巍1 陈郁希2 李强3△ 李学超1 阚毅1.胃转流术后远端肠黏膜形态及生长因子表达的变化[J].,2011,11(18):3446-3448 |
| 胃转流术后远端肠黏膜形态及生长因子表达的变化 |
| Changes in Morphology of Intestinal Mucosa and Expressions of GrowthFactors after Gastric Bypass Surgery in Rats |
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| DOI： |
| 中文关键词: 胃转流术 表皮生长因子 胰岛素样生长因子-1 肠粘膜 |
| 英文关键词: Gastric bypass surgery Epidermal growth factor Insulin-like growth factor 1 Mucosa |
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| 中文摘要: |
| 目的：探讨胃转流术后远端肠管黏膜的适应性变化及生长因子的表达情况。方法：将8 周龄的Wistar 大鼠随机分为对照组、
假手术组和胃转流术组，术后8 周取吻合口远端肠管行常规病理切片检查，测量肠黏膜厚度和绒毛高度，采用免疫荧光法检测肠
粘膜中表皮生长因子(EGF)和胰岛素样生长因子-1(IGF-1)的表达。结果：胃转流术组黏膜厚度(672±39 与500±31μm，P<0.01)
和绒毛高度(445± 19 与342±15μm，P<0.01)均显著高于假手术组(P<0.01)，而对照组和假手术组间均无显著差异。与假手术组
比较，胃转流术组肠粘膜中EGF 和IGF-1 的表达显著升高(P<0.01)，而对照组和假手术组间无显著差异。结论：胃转流术后远端肠
管粘膜发生适应性增生，同时伴随着EGF 和IGF-1 表达水平的升高。 |
| 英文摘要: |
| Objective: To investigate the changes in the morphology of intestinal mucosa and the expressions of growth factors
after gastric bypass surgery (GBP). Methods: Wistar rats (eight-week old) were divided into three groups: control group, sham group, and
GBP group. The distal bowel was collected after 8 weeks following the surgery. The mucosal thickness and villus height were measured
by histological analysis. The expressions of epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) were detected by
immunofluorescence. Results: The mucosal thickness (672 ± 39 vs. 500 ± 31μm, P<0.01) and villus height (445 ± 19 vs. 342 ±
15μm, P<0.01) in GBP group were significantly higher than sham group. In addition, the mucosal thickness and villus height were similar
between control group and sham group. Compared with sham group, the expressions of EGF and IGF-1 were significantly increased (both
P<0.01). Additionally, the expressions of EGF and IGF-1 were similar between control group and sham group. Conclusion: Interstinal
mucosal adaptation occurred in distal bowel, which is associated with increased expressions of EGF and IGF-1. |
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