| 韩怡1 任强2 郭道森1 刘毓3 李荣贵1.一种崂山百合的离体快速繁殖技术[J].,2011,11(18):3453-3457 |
| 一种崂山百合的离体快速繁殖技术 |
| A rapid propagation method for Lilium tsingtauense Gilg |
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| DOI： |
| 中文关键词: 崂山百合 组织培养 快速繁殖 |
| 英文关键词: Lilium tsingtauense Gilg Tissue culture Propagation |
| 基金项目:济科合字2008D07061；山东省科技攻关项目（2010GG0020937） |
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| 中文摘要: |
| 以崂山百合的鳞茎为外植体，通过植物组织培养技术成功获得了再生植株，建立了该植物的快速无性繁殖体系。整个组织
培养过程分为芽的诱导、根的诱导和移栽三个阶段。适合芽诱导的最佳外植体是：鳞茎基部，最适培养基是：1/2 MS + 0.5 mg/L
NAA + 0.8 mg/L KT + 3 %蔗糖。用于生根的最佳培养基是：1/2 MS + 0.4 mg/L IBA + 3 %蔗糖。最适培养温度为25℃.不同基质对
组培苗移栽的成活率有一定影响，其中在珍珠岩上的移栽成活率为98 %。 |
| 英文摘要: |
| Different parts of bulb from Lilium tsingtauense Gilg were used as explants to regenerate seedlings by tissue culture.,
and a rapid propagation method was established. The culture process could be constituted of three stages, which were induction of buds,
induction of roots and transplant. The optimal medium for induction of buds was 1/2 MS + 0.5 mg/L NAA + 0.8 mg/L KT + 3 %sucrose,
while the optimal medium for induction of roots was 1/2 MS + 0.4 mg/L IBA + 3 %sucrose. The best culturing temperature was 25 ℃.
Different transplant mediums had some effects on the survival rate of regenerated seedlings, and the survival rate of regenerated seedlings
on perlite was 98%. |
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