| 杨江河1 张蓉2 李华1 修彬华1 刘绍明1.模拟微重力促进骨髓间充质干细胞神经营养因子的表达[J].,2011,11(20):3857-3859 |
| 模拟微重力促进骨髓间充质干细胞神经营养因子的表达 |
| Modeled-Microgravity Increases the Expression of Neurotrophins in MSCs |
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| DOI： |
| 中文关键词: 微重力 骨髓间充质干细胞 神经营养因子 |
| 英文关键词: Microgravity MSCs Neurotrophins |
| 基金项目: |
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| 中文摘要: |
| 目的：探究微重力对干细胞向神经元分化的影响, 为临床治疗神经退行性疾病提供新的思维和方法。方法：运用流式细胞术
鉴定骨髓间充质干细胞及其微重力对凋亡的影响。运用反转录PCR 检测微重力干预干细胞48h 及72h 后神经营养因子BDNF,
CNTF,NGF 的表达。运用ELISA 检测其分泌营养因子的浓度。结果：流式细胞仪检测细胞凋亡, 两组之间并无差异。CNTF 分泌
在微重力干预后有明显增加(p<0.05)。BDNF 浓度有轻度增加。结论：模拟微重力可以促进骨髓间充质干细胞神经营养因子的表
达。 |
| 英文摘要: |
| Objective: To investigate the effects of microgravity on the differentiation of MSCs into neurons. Method: Flow
cytometry analysis on the cells of passage 2 was used to identify MSCs. RT-PCR was used to detect the neurotrophins expression in
MSCs following microgravity stimulation. The neurotrophins levels in MSCs were detected by ELISA. Results: Compared with that in 1g
group, the expression of CNTFand BDNF after MG stimulation was higher. Conclusion: Modeled-microgravity increased the expression
of neurotrophins in MSCs. |
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