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闫文1 杨银书1 卢娟1.HSP70 高表达保护低氧诱导的大鼠海马DG 区神经细胞凋亡[J].,2012,12(1):27-29 |
| HSP70 高表达保护低氧诱导的大鼠海马DG 区神经细胞凋亡 |
| HSP70 Over Expression Protected Neurocyte in the Area of DentateGyrus（DG）of Rat's Hippocampus From Hypoxia-Induced Apoptosis |
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| DOI： |
| 中文关键词: 热休克蛋白70 低氧 细胞凋亡 |
| 英文关键词: HSP70 Radiation Apoptosis |
| 基金项目:全军重点基金课题（G-867） |
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| 中文摘要: |
| 目的：研究诱导HSP70 高表达对低氧引起的大鼠海马DG 区神经细胞凋亡的保护作用。方法：大鼠海马DG 区神经细胞分
别在41℃温浴2h 和加入砷酸钠诱导HSP70 高表达。对照低氧而不预热，并用HSP70 反义寡核苷酸链抑制HSP70 合成，观察
HSP70 与低氧大鼠海马DG 区神经细胞凋亡的关系。DNA 碎片法检测细胞凋亡，Western Blotting 检测HSP70。结果：预热和砷酸
钠都可以诱导细胞HSP70 高表达；HSP70 高表达可以明显减少低氧诱导的细胞凋亡。在预热前导入HSP70 反义核酸，可以降低
HSP70 抑制细胞凋亡的作用。结论：HSP70 高表达可以保护细胞由于低氧引起的细胞凋亡。 |
| 英文摘要: |
| Objective: To investigate the effect of the over expression of HSP70 on neurocyte in the area of dentate gyrus（DG）of
rat's hippocampus in hypoxia-induced apoptosis. Methods: Cells were either heated in 41℃ water for 2 hours or affected with 10μmol/L
sodium arsenite for 18h to induced the over expression of HSP70. While the control cells are added HSP70 antisense oligomer which reduced
the HSP70 expression or not induced before the cells heated. Then the cells were incubated in hypoxia. Fragmentation of DNA
was quantities, and HSP70 was analyzed at various times by the Western blot. Results: Both heat and sodium arsenite can induce the over
expression of HSP70, which can significantly decreased hypoxia-induced apoptosis. Introduction of the HSP70 antisense oligomer prior
to heating reserved the protective effect of HSP70. Conclusions: The over expression of HSP70 can protect the pre-blood cells from radiation-
induced apoptosis. |
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