| 王峥1 聂同钢2 李敬永3.STR 分型应用于病理标本污染的个体识别[J].,2012,12(1):86-89 |
| STR 分型应用于病理标本污染的个体识别 |
| Application of Individual Identification through PCR-STR for IdentifyingContaminated Pathological Samples |
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| DOI： |
| 中文关键词: 浆液性囊腺癌 标本污染 DNA 提取 STR 分型 |
| 英文关键词: Cystadenocarcinoma Contaminated samples DNA extraction STR typing |
| 基金项目: |
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| 摘要点击次数: 714 |
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| 中文摘要: |
| 目的：通过STR 分型，对两例卵巢囊腺癌病理标本的组织细胞进行个体识别，以鉴别其中一例病理标本癌细胞是否源于污
染。方法：对甲醛固定石蜡包埋组织，分别切片后在倒置显微镜下，用显微操作系统分离出不同形态的细胞团，对提取的DNA 样
本进行常规STR 扩增检验。结果：经过STR 分型比对，证实病理标本存在污染，其中一例的癌细胞来源于另一个体。结论：STR 分
型是用于病理石蜡包埋组织标本污染个体识别的有效手段。 |
| 英文摘要: |
| Objective: To use the STR typing to identify the two pathological samples of ovary cystadenocarcinoma, in order to
identify whether one of two pathological samples was contaminated. Methods: Paraffin-embedded tissues were sliced and the different
morphological cells were isolated by micro-operating system on inverted microscope. Extract DNA of the isolated cells, and get the genotype
through regular PCR-STR method. Results: The genotype show the existence of contamination of the pathological samples and
prove that cancer cells of sample were originated from the other sample. Conclusion: Individual identification through the PCR-STR
method was the effective method to identify the contaminated pathological slice. |
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