| 吴爱兵1 邓旭斌2 杨志雄1 沈湘1 黎明春1 李姝君1 吴昆鹏1.鼻咽癌ALDH1+细胞具有上皮细胞- 间质转化的性质*[J].,2014,14(20):3819-3822 |
| 鼻咽癌ALDH1+细胞具有上皮细胞- 间质转化的性质* |
| ALDH1 Positive Cells Posses the Properties of Epithelial-Mesenchymal Transition* |
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| DOI： |
| 中文关键词: 上皮细胞- 间质转化 乙醛脱氢酶1 鼻咽癌 肿瘤干细胞 |
| 英文关键词: Epithelial-Mesenchymal Transition ALDH1 Nasopharyngeal carcinoma Cancer StemCell |
| 基金项目:国家自然科学基金项目（81201672）；湛江市科技攻关计划（2012C3104032） |
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| 中文摘要: |
| 摘要目的：探讨鼻咽癌ALDH1+细胞是否具有上皮细胞- 间质转化的性质。方法：利用ALDEFLUOR 试剂分选鼻咽癌ALDH1+
细胞和ALDH1- 细胞，Transwell 及Boyden 小室实验分析ALDH1+细胞和ALDH1-细胞的侵袭、迁移能力；实时定量PCR 及
Western blot 检测E-cadherin、Vimentin、Snail 及Twist 基因在ALDH1+细胞和ALDH1-细胞mRNA 和蛋白表达水平。结果：与
ALDH1-细胞相比，ALDH1+细胞侵袭及迁移能力明显高于ALDH1-细胞；而且ALDH1+细胞中E-cadherin 的mRNA和蛋白水平
明显低于ALDH1-细胞，相反，ALDH1+细胞中Vimentin、Snail及Twist 的mRNA 和蛋白水平明显高于ALDH1-细胞。结论：鼻咽
癌ALDH1+细胞可能具有上皮- 间质转化的性质，这将为阐明肿瘤细胞在侵袭、转移中的机制提供理论基础。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the effect of ALDH1-positive cells on the properties of epithelial Mesenchymal Transition.
Methods:The identification of ALDH1 activity from 5-8F and CNE2 cells using the Aldefiuor assay (Stem Cell Technologies, Durham,
NC, USA) was followed by fiuorescence-activated cell sorting analysis. The ability of ALDH1-positive and ALDH1-negative cells to
migrate and metastasize was detected by Transwell and Boyden in vitro. The expressions of E-cadherin, Vimentin, Snail and Twist were
evaluated by real-time PCR and Western blot. Results:Compared with that in ALDH1-negative cells, ALDH1-positive cells showed
higher invasion and metastasis activity. The expression of E-cadherin was lower in ALDH1- positive cells than that in ALDH1-negative
cells, in contrast, the expression of Vimentin, Snail and Twist were higher in ALDH1-positive cells than that in ALDH1-negative cells.
Conclusion:ALDH1 positive cells may have the properties of epithelial-mesenchymal transition. It will provide a new experimental
theoretical basis for invasion and metastasis of nasopharyngeal carcinoma. |
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