| 杨蕾 薛琳 陈景荷 赵国宏 苏婕 张德新.血清应答因子在食管鳞癌细胞侵袭转移中的作用[J].,2014,14(30):5810-5813 |
| 血清应答因子在食管鳞癌细胞侵袭转移中的作用 |
| The Effect of SRF on the Invasion and Metastasis of HumanEsophageal Squamous Cell Carcinoma |
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| DOI： |
| 中文关键词: 食管鳞癌 SRF 侵袭 转移 |
| 英文关键词: Esophageal squamous cell carcinoma SRF Invasion Metastasis |
| 基金项目:国家自然科学基金面上项目(81272347) |
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| 摘要点击次数: 956 |
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| 中文摘要: |
| 目的：研究血清应答因子（serum response factor，SRF）在人食管鳞癌细胞体外侵袭转移中的意义。方法：选用EC9706-H、
EC9706-L和EC109-H、EC109-L两对高低转移细胞系，采用细胞划痕实验验证食管鳞癌高低转移细胞系体外侵袭转移能力的差
异；Western blot检测SRF在两对食管鳞癌高低转移细胞系中的差异表达；在EC9706-H、EC109-H 细胞中加入CCG（SRF 抑制
剂）抑制SRF的表达后，检测其侵袭转移能力的变化。结果：细胞划痕实验验证了两对食管鳞癌高低转移细胞系侵袭转移能力的
差异；Western blot 结果提示SRF 在EC9706-H、EC109-H 细胞中的表达水平显著高于EC9706-L、EC109-L 细胞；在EC9706-H、
EC109-H 细胞中加入CCG抑制SRF的表达后，其侵袭转移能力明显减退。结论：SRF在高转移性食管鳞癌细胞系中呈现高表达，
在低转移性食管鳞癌细胞系中呈现低表达，抑制高转移性食管鳞癌细胞系中SRF 的表达后，其侵袭转移能力下降，提示SRF 和
食管鳞癌的侵袭转移能力呈正相关。 |
| 英文摘要: |
| Objective:The aimof this study was to investigate the in vitro effect of serum response factor (SRF) on the invasion
and migration of esophageal squamous cell carcinoma (ESCC) in vitro.Methods:The invasion and migration of ESCC cell lines were
detected by scratch assays. The expression of SRF in ESCC cell lines were measured by western blot. CCG was used to suppress the
expression of SRF in highly invasive cells of ESCC.Results:There were differences with the invasion and migration in the ESCC cell
lines. The expression of SRF in highly invasive cells of ESCC was higher than the matched lowly invasive cells. After treatment with
CCG, the invasion and migration of highly invasive cells were obviously decreased.Conclusion:The expression level of SRF may be
positively correlated to the invasion and migration of ESCC cell lines, and SRF may be a novel molecular marker for the prediction of
migration, diagnosis and therapy of ESCC. |
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