| 陈泽华 付彩云 刘俊 黎其友 阴正勤.表皮生长因子诱导骨髓间充质干细胞向视网膜神经细胞分化的体外研究[J].,2014,14(30):5818-5820 |
| 表皮生长因子诱导骨髓间充质干细胞向视网膜神经细胞分化的体外研究 |
| Experimental Research on the Differentiation of Human Bone MesenchymalStemCells into Retinal Cells in vitro |
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| DOI： |
| 中文关键词: 骨髓间充质干细胞 视网膜神经细胞 体外分化 |
| 英文关键词: Bone marrow mesenchymal stemcells Retina Cellular differentiation |
| 基金项目:国家重点基础研究发展计划（973计划）(2013CB967001) |
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| 中文摘要: |
| 目的：研究表皮生长因子诱导骨髓间充质干细胞向视网膜神经细胞分化的可能性。方法：体外培养骨髓间充质干细胞，利用
流式细胞仪分析其细胞表型。采用含EGF的培养液诱导骨髓间充质干细胞向视网膜神经细胞分化，并利用免疫荧光法进行鉴定。
结果：从骨髓中分离培养的细胞具有成纤维细胞样形态，贴壁生长，表型相对均一，表面标志为CD90、CD44、CD147阳性；而
CD34、CD38、CD45、CD14、HLA-DR 阴性。体外诱导后可以得到神经干细胞标志物nestin、神经胶质细胞标志物GFAP 和视网膜
光感受器细胞标志物Rhodopsin 呈阳性表达的细胞。结论：从骨髓中分离培养得到的间充质干细胞具有向视网膜神经细胞分化的
潜能。 |
| 英文摘要: |
| Objective:To study the differentiation of human bone marrow-derived mesenchymal stemcells into retinal cells in vitro.Methods:HMSCs were isolated fromhuman bone marrow after Ficoll density gradient centrifugation. The adherent cells after cultivation
at least 3 passages were used for study. Immunophenotype of the cells was analyzed by Flow cytometer, and cellular differentiation
was identified by immunofluorescence labeling technique.Results:The target cells derived from human bone marrow adhered to the
plate with fibroblastic-like morphology, whose surface markers were similar as mesenchymal stem cells. Major cells were positive for
CD90, CD44 and CD147, while they were all negative for CD34, CD45 and HLA-DR. In the differentiation study, HMSCs were cultured
in induced medium can differentiate into nestin positive cell, GFAP positive glial cells and retina-specific neurons expressing Rhodopsin
with CD90 negative.Conclusion:HMSCs are capable of differentiating into the retinal neural cells in vitro. |
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