| 张伟辉 乔昕 吕航 马文超 王端平 高博 孙旺.饥饿及雨蛙素诱导的AR42J 细胞中自噬基因LC3及beclin-1 表达的变化[J].,2015,15(8):1415-1417 |
| 饥饿及雨蛙素诱导的AR42J 细胞中自噬基因LC3及beclin-1 表达的变化 |
| Changes of LC3 and Beclin 1 Expression in the Starvation and Ceruleininduced AR42J Cells |
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| DOI： |
| 中文关键词: 胰腺炎 Beclin-1 微管相关蛋白LC3 自噬性程序性细胞死亡 胰腺腺泡细胞 |
| 英文关键词: Acute pancreatitis Beclin-1 Microtubule associated protein LC3 Autophagy programmed cell death |
| 基金项目:黑龙江省教育厅科学技术研究基金项目(12511242) |
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| 中文摘要: |
| 目的：观察饥饿及雨蛙素诱导的大鼠胰腺腺泡细胞AR42J 中自噬基因LC3 及beclin-1 表达的变化，初步探讨吞噬
(autophagy)在急性胰腺炎中的作用。方法：选择体外培养的生长状态良好的大鼠胰腺腺泡AR42J 细胞，随机分为3 组，饥饿组
(N=10)，雨蛙素处理组(N=10)，空白对照组(N=10)。饥饿组加入充足的平衡盐溶液，雨蛙素处理组加入含10-7mol/L雨蛙素的全营
养培养液，空白对照组加入含20%灭活胎牛血清的F12-K 培养液(pH7.2-7.4)，各组分别于处理后2、4、6 h收集细胞并提取蛋白
质。采用免疫印迹法检测三组不同时点胰腺腺泡细胞AR42J 中自噬基因Beclin-1 和LC3 的蛋白表达。结果：空白对照组不同时点
beclin-1 和LC3-II均呈低表达，且各时点比较差异无统计学意义(P<0.05)。饥饿组和雨蛙素处理组beclin-1 和LC3-II 的表达随处
理时间的延长逐渐增加，且不同时点beclin-1 和LC3-II的表达均较空白对照组显著增高，差异均具统计学意义(P<0.05)。结论：雨
蛙素和饥饿刺激可导致大鼠胰腺腺泡细胞AR42J 中LC3-II及beclin-1 蛋白表达随作用时间的延长而增加，自噬可能参与了胰腺
炎的发生发展过程。 |
| 英文摘要: |
| Objective:To discuss the effect of phagocytosis(autophagy) in patients with acute pancreatitis by observing the change
of the expression of LC3 and beclin-1 gene in starvation and caerulein induced AR42J rat pancreatic acinar cells.Methods:The growth
state of good rat pancreatic acini AR42J cells in vitro were randomly divided into 3 groups, starvation group (N = 10), caerulein treated
group (N = 10), blank control group (N = 10). Balanced salt solution sufficient to join the starvation group, caerulein treated group added
with fullnutrient solution containing 10-7 mol/L of caerulein, blank control group added with inactivated containing 20% fetal bovine
serum F12 - K nutrient solution (pH7.2-7.4), all the group collected cells and extracted proteins at 2, 4, 6 h respectively after treatment.
Detected the expression of protein of autophagy genes Beclin-1 and LC3 by Western blot in each groups at different time points of
ARJ42J pancreatic acinar cells.Results:The blank control group at different tim points of beclin-1 and LC3-Ⅱ showed low expression
and no significant different at each time point (P<0.05). The expression of beclin-1 and LC3-Ⅱ were increased gradually with the
prolonged time in starvation and caerulein treatment group, and both of them were significantly increased compared with blank control
group at different time points, the difference was statistically significant (P<0.05).Conclusion:Caerulein and starvation can lead to
increase of the expression of LC3-Ⅱ and beclin-1 in AR42J rats pancreatic acinar cells with the prolongation of time,autophagy may
participate in the occurrence and development of pancreatitis. |
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