| 张炼 钱济先 刘永明 裘秀春 马保安.退变腰椎间盘中IL-17表达的变化及其意义[J].,2015,15(8):1457-1460 |
| 退变腰椎间盘中IL-17表达的变化及其意义 |
| The Changes of IL-17 Expression in Degenerated Human LumbarIntervertebral Disc and its Significance |
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| DOI： |
| 中文关键词: 椎间盘退变 Th17 细胞 IL-17 RORγt |
| 英文关键词: Intervertebral disc Th17 cell IL-17 RORγt |
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| 中文摘要: |
| 目的：研究退变的椎间盘组织中IL-17 表达的变化及其与椎间盘退变严重程度之间的关系。方法：收集退变椎间盘标本23
例，正常椎间盘标本12 例，通过免疫组织化学染色、免疫荧光染色、实时- 定量PCR(RT-PCR)和酶联免疫吸附实验(ELISA)从细
胞、蛋白和基因水平检测椎间盘组织中IL-17 和孤独受体(retinoid-related orphan receptor，RORγt )的表达。结果：免疫组化染色显
示退变椎间盘组织中IL-17 阳性细胞比例较对照组明显增高，有统计学差异(P<0.05)；免疫荧光染色显示退变椎间盘组织中Th17
细胞含量明显增多(P<0.05)；退变的椎间盘组织中IL-17 和RORγt mRNA的相对表达量较对照组增加，有统计学差异(P＜0.001)，
且两者之间呈显著正相关(r=0.6919，P＜0.001)；退变的椎间盘组织中IL-17 的含量较对照组明显增加(P<0.01)，且与椎间盘退变的
严重程度呈正显著相关(r=0.4714，P＜0.01)。结论：IL-17 含量增加参与了腰椎间盘退变的病理过程，并且可能对椎间盘退变起促
进作用。 |
| 英文摘要: |
| Objective:To investigate the expression of IL-17 in degenerated human lumbar intervertebral disc tissues, and explore
the relationship between IL-17 expression and severity of intervertebral disc degeneration.Methods:The level of IL-17 protein and Th17
cells were respectively determined by immunohistochemistry and immunofluorescence in 35 surgical lumbar intervertebral disc tissues
(23 samples from patients with lumbar disc degeneration and 12 non-degenerated control tissues from patients with lumbar acute
fractures). IL-17 and retinoid-related orphan receptor (RORγt) mRNA levels were respectively measured by real-time PCR (RT-PCR)
and ELISA.Results:The percentage of IL-17(+) cells was significantly higher than that of the control group(P<0.05). Immunofluorescent
staining showed that the percentage of Th17 (+) cells in degenerated human lumbar intervertebral disc tissues was evidently increased
(p<0.05). The mean IL-17 and RORγt mRNA levels in degenerated disc tissues group were significantly higher than those in the
non-degenerated controls (P＜0.001). ELISA results showed IL-17 expression was significantly enhanced in degenerate human lumbar
intervertebral disc tissues, compared with the non-degenerated control tissues (P<0.01). The expression level of IL-17 mRNA was
evidently higher than that of the control group(P<0.01), and was positively correlated with the severity of intervertebral disc degeneration
(r=0.4714, P<0.01).Conclusion:The increase of IL-17 expression was involved in the pathological process of lumbar disc degeneration,
which may contribute to the incidence of lumbar disc degeneration. |
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