| 杨培青,汪 云,梅夏齐,王风秀.藏红花酸对TGF-β1刺激的人肝星状细胞LX-2信号转导通路的影响[J].,2018,(17):3230-3234 |
| 藏红花酸对TGF-β1刺激的人肝星状细胞LX-2信号转导通路的影响 |
| Effects of Crocetin on Signal Transduction Pathway of Human Hepatic Stellate Cells Stimulated by TGF-β1 |
| 投稿时间：2018-01-03 修订日期：2018-01-30 |
| DOI：10.13241/j.cnki.pmb.2018.17.006 |
| 中文关键词: 肝星状细胞 转化生长因子 藏红花酸 TGF-β1/Smads |
| 英文关键词: Hepatic stellate cell Transforming growth factor-β1 Crocetin TGF- β1/Smads |
| 基金项目:黑龙江省自然科学基金项目（D201076） |
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| 中文摘要: |
| 摘要 目的：研究藏红花酸（crocetin）对转化生长因子-β1（TGF-β1）刺激的人肝星状细胞LX-2信号转导通路的影响。方法：体外培养LX-2细胞，随机设立空白对照组，模型组，藏红花酸低剂量组，藏红花酸中剂量组，藏红花酸高剂量组，用含10%血清的1640培养液培养48 h，MTT法测各组细胞增殖，蛋白免疫印迹测定各组细胞α-SMA蛋白的表达，实时荧光定量PCR法测各组细胞Smad2、Smad3、Smad7mRNA的表达。结果：与对照组比较，TGF-β1刺激后人肝星状细胞LX-2增殖作用明显，且上调α-SMA蛋白、Smad2mRNA、Smad3mRNA表达，下调Smad7mRNA表达（P<0.01）与模型组比较，藏红花酸组均能抑制LX-2细胞增殖，并呈剂量依赖性，高、中剂量组作用明显，能够明显下调α-SMA蛋白、Smad2mRNA、Smad3mRNA表达，上调Smad7mRNA表达，差异具有统计学意义（P<0.05）。结论：藏红花酸对TGF-β1刺激的LX-2细胞中Smad7mRNA具有上调作用，对Smad2、Smad3mRNA具有下调作用，其抗肝纤维化作用可能与抑制TGF-β1/Smads信号转导通路有关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects of crocetin on TGF-β1 stimulated signal transduction pathway in human hepatic stel- late cells (HSC-LX2). Methods: HSC-LX2 cells were cultured in vitro and randomly divided into 5 groups: blank group, model group, low-dose crocetin group, medium-dose group, high-dose crocetin group. Then cells proliferation were measured by MTT, the expression of α-SMA was measured by Western blot, the gene expression of Smad2/Smad3/Smad7 mRNA were measured by Real-time PCR. Results: Compared with control group, the model group promotes cells proliferation. It can upregulate the expression of α-SMA protein, Smad2mRNA and Smad3mRNA and downregulate the expression of Smad7mRNA (P<0.01). Compared with model group, crocetin group can inhibit the effect of TGF-β1 in a dose-dependent manner(P<0.05). The high-dose and medium-dose crocetin groups can down- regulate the expression of α-SMA protein, Smad2mRNA and Smad3mRNA and upregulate the expression of Smad7mRNA (P<0.05). Conclusion: Crocetin can upregulate the expression of Smad7mRNA and downregulate the expression of Smad2mRNA and Smad3mR- NA in LX-2 cells induced by TGF-β1. Thus the anti-hepatic fibrosis mechanism of crocetin may be related to the inhibition of TGF-β1/Smads signal transduction pathway. |
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