| 徐自慧,王雅蕾,万亮琴,何 芳,陈紫微,王 雪,宋晨晨,谭 琰,王 旭,董瑞娟,葛东宇,华 茜.肝癌小鼠外周血及脾脏T淋巴细胞亚群的变化及其意义[J].,2019,19(8):1406-1409 |
| 肝癌小鼠外周血及脾脏T淋巴细胞亚群的变化及其意义 |
| The Changes of T Cell Subsets in Peripheral Blood and Spleen and Its Significance in Hepatoma Mice |
| 投稿时间：2018-08-08 修订日期：2018-08-31 |
| DOI：10.13241/j.cnki.pmb.2019.08.002 |
| 中文关键词: 小鼠原位肝癌模型 T淋巴细胞亚群 流式细胞术 |
| 英文关键词: Orthotopic liver cancer model T cell subsets Flow cytometry |
| 基金项目:国家自然科学基金项目（81473546）；藏医药区域协同创新中心项目（2017XTCX012）；中央高校基本科研业务费专项基金资助（2018-JYBZZ-XJSJJ011） |
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| 中文摘要: |
| 摘要 目的：观察小鼠原位肝癌模型外周血以及脾脏T淋巴细胞亚群与正常小鼠之间的差异变化，探讨其差异变化的意义。方法：在正常KM小鼠肝脏种植H22细胞，建立小鼠原位模型。采用流式细胞术，以健康正常小鼠为对照，检测肝癌小鼠外周血以及脾脏T淋巴细胞亚群的变化。结果：与健康正常小鼠相比，肝癌小鼠外周血CD4+T淋巴细胞、CD4+/CD8+比例有显著性降低，CD8+T淋巴细胞显著性升高；脾脏CD3+、CD4++T淋巴细胞有显著性降低。结论：小鼠原位肝癌模型外周血以及脾脏T淋巴细胞亚群发生异常，免疫系统紊乱，可以反映小鼠肝癌的发生、发展。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the difference of T cell subsets in peripheral blood and spleen between normal mice and orthotopic liver cancer model, and to explore the significance of the difference. Methods: H22 cells were implanted in the liver of KM mice to establish hepatoma mice and flow cytometry was used to test the changes of T cell subsets in peripheral blood and spleen in hepatoma mice and normal mice as control. Results: Compared with normal mice, the proportion of CD4+ T cells and CD4+/CD8+ in peripheral blood of hepatoma mice was significantly decreased, well, the proportion of CD8+ T cells significantly increased. The proportion of CD3+, CD4++ T cells in spleen was significantly reduced. Conclusion: After H22 cells were implanted in the liver of mice, the T cell subsets in peripheral blood and spleen were abnormal, and the immune system was disordered. It can reflect the occurrence and development of liver cancer in mice. |
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