| 刘瑞廷,侯亚莉,白继荣,王国荣,邱冷健,阎立昆,李小军,王小强.UBE2T基因对结直肠细胞增殖和凋亡的影响[J].,2019,19(15):2834-2838 |
| UBE2T基因对结直肠细胞增殖和凋亡的影响 |
| Effect of UBE2T Gene on the Proliferation and Apoptosis of Colorectal Cells |
| 投稿时间：2018-12-08 修订日期：2018-12-30 |
| DOI：10.13241/j.cnki.pmb.2019.15.007 |
| 中文关键词: UBE2T 结直肠癌 增殖 凋亡 |
| 英文关键词: UBE2T Colorectal cancer Proliferation Apoptosis |
| 基金项目:陕西省国际科技合作与交流计划项目(2017KW-046) |
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| 中文摘要: |
| 摘要 目的：探讨人类泛素结合酶E2T(Ubiquitin-conjugating enzyme E2T，UBE2T)基因对结肠细胞增殖和凋亡的影响。方法：体外培养人正常结直肠粘膜细胞FHC，采用将UBE2T基因慢病毒质粒转染至FHC细胞48 h后，通过MTT法检测细胞增殖情况，western blotting检测细胞中增殖相关蛋白UBE2T蛋白、Ki67、促凋亡蛋白Bax和抗凋亡蛋白Bcl-2的表达，流式细胞术检测细胞凋亡率。结果：与转染空质粒的FHC细胞相比，UBE2T基因慢病毒质粒转染FHC细胞48 h后，细胞增殖能力显著上调(P<0.05)，UBE2T蛋白明显增加，Ki67的表达明显增加(P<0.05)，细胞凋亡率显著降低(P<0.05)，且Bax的表达明显下调而Bcl-2的表达上调(P<0.05)。结论：UBE2T基因能够促进正常结肠粘膜细胞的增殖，并抑制其凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effects of UBE2T(Ubiquitin-conjugating enzyme E2T) gene on the apoptosis of colorectal cells. Methods: Normal colorectal mucosal cells FHC were cultured in vitro and transfected by UBE2T lentivirus plasmid for 48 h, MTT was used to detect the cell proliferation; the UBE2T protein, the proliferation-associated protein Ki67, the pro-apoptotic protein Bax and the anti-apoptotic protein Bcl-2 in cells were detected by Western Blotting; the apoptosis rate of cells was evaluated by Flow cytometry (FCM). Results: Compared with FHC cells transfected with empty plasmid, the proliferation ability of FHC cell transfected by UBE2T lentivirus plasmid was obviously upregulated (P<0.05), the expression of UBE2T protein and Ki67 protein were significantly increased, the apoptosis rate was obviously decreased, the expression of Bax protein was significantly decreased and the expression of Bcl-2 protein was obviously increased(P<0.05). Conclusion: UBE2T gene promoted the proliferation and inhibited the apoptosis of normal colorectal cells FHC. |
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